CAJ | 학술논문

为确定烟草转录因子NtWRKY-R1的转录激活域,本研究将NtWRKY-R1的编码区分为7个不同片段,分别构建到酵母表达载体pGBKT7中与GAL4的DNA结合域融合,通过酵母双杂交试验确定,NtWRKY-R1本身具有能够激活GAL4 BD转录起始的激活结构域,并将转录激活区定位于该转录因子蛋白肽链的N端75个氨基酸残基和C端80个氨基酸残基中。
위학정연초전록인자NtWRKY-R1적전록격활역,본연구장NtWRKY-R1적편마구분위7개불동편단,분별구건도효모표체재체pGBKT7중여GAL4적DNA결합역융합,통과효모쌍잡교시험학정,NtWRKY-R1본신구유능구격활GAL4 BD전록기시적격활결구역,병장전록격활구정위우해전록인자단백태련적N단75개안기산잔기화C단80개안기산잔기중。
To identify the activation domain of tobacco transcription factor NtWRKY-R1,coding region of NtWRKY-R1 was separated to seven fragments and then were fused with GAL4 BD ( DNA binding do-main) on pGBKT7 vector in this work. The yeast two hybridization assay showed that NtWRKY-R1 could activate the transcription initiation of GAL4 BD,and the activation domain of NtWRKY-R1 was localized in 75 residues at N-terminus and 80 residues at C-terminus of NtWRKY-R1 .