CAJ | 학술논문

以拟南芥和水稻21个纤维素合成酶基因( CesA)为研究对象,对其基因结构、保守结构域、蛋白质跨膜结构、亚细胞定位、基因表达等进行综合分析。结果显示,CesA基因长度在3.5~6.5 kb,有7~13个内含子( OsCesA10除外)。 CesA基因编码的蛋白质保守性较强,含有该基因家族的保守结构域Ring finger( OsCesA10、OsCesA11除外)和Cellulose synthase。水稻和拟南芥的CesA蛋白跨膜螺旋数量为6个或8个( OsCesA10除外)。蛋白质亚细胞定位结果表明,21条CesA蛋白全部定位在质膜上。大部分AtCesA基因在植株的幼根、幼叶和愈伤组织中均有表达,而大部分OsCesA基因仅在幼根或幼叶中表达。
이의남개화수도21개섬유소합성매기인( CesA)위연구대상,대기기인결구、보수결구역、단백질과막결구、아세포정위、기인표체등진행종합분석。결과현시,CesA기인장도재3.5~6.5 kb,유7~13개내함자( OsCesA10제외)。 CesA기인편마적단백질보수성교강,함유해기인가족적보수결구역Ring finger( OsCesA10、OsCesA11제외)화Cellulose synthase。수도화의남개적CesA단백과막라선수량위6개혹8개( OsCesA10제외)。단백질아세포정위결과표명,21조CesA단백전부정위재질막상。대부분AtCesA기인재식주적유근、유협화유상조직중균유표체,이대부분OsCesA기인부재유근혹유협중표체。
In this study,twenty-one cellulose synthase genes( CesA) from Arabidopsis and rice were ana-lyzed in their structures,conservative motifs,protein transmembrane helices,protein subcellular locations and gene expressions. The results showed that the size of CesA ranged from 3. 5 to 6. 5 kb,with 7—13 small introns(except OsCesA10). CesA had highly conserved structure with the family domain “Ring fin-ger”( except OsCesA10 and OsCesA11 ) and “Cellulose synthase”. The number of protein transmembrane helices in Arabidopsis and rice was 6 or 8 ( except OsCesA10 ) , respectively. The subcellular locations showed that the 21 CesA proteins were all located on the membranes. Most of the AtCesA genes expressed in young root, young leaves and calluses, whereas most of the OsCesA expressed only in young root or young leaves.