CAJ | 학술논문

目的：观察针刺干预对脑缺血大鼠脑组织缺氧诱导因子1α（ hypoxia－inducible factor－1α，HIF－1α）蛋白及mRNA表达的影响，探讨针刺对缺血性脑血管疾病的防治机制。方法建立局灶性脑缺血大鼠模型，随机分为正常组、假手术组、模型组、针刺组（各8只大鼠），应用蛋白印迹及实时荧光定量检测针刺对局灶性脑缺血大鼠脑组织HIF－1α蛋白及mRNA表达的变化。结果正常组和假手术组大鼠比较HIF－1α蛋白及mRNA表达无明显变化，模型组大鼠HIF－1α蛋白及mRNA表达上调，差异无统计学意义；针刺组大鼠HIF－1α蛋白及mRNA表达高于模型组（0．567±0．058 vs 0．315±0．118；1．593±0．102 vs 1．193±0．259），差异有统计学意义（P＜0．05）。结论脑缺血后大鼠脑组织HIF－1α蛋白及mRNA表达增强，针刺可以上调其表达从而发挥脑保护作用。
목적：관찰침자간예대뇌결혈대서뇌조직결양유도인자1α（ hypoxia－inducible factor－1α，HIF－1α）단백급mRNA표체적영향，탐토침자대결혈성뇌혈관질병적방치궤제。방법건립국조성뇌결혈대서모형，수궤분위정상조、가수술조、모형조、침자조（각8지대서），응용단백인적급실시형광정량검측침자대국조성뇌결혈대서뇌조직HIF－1α단백급mRNA표체적변화。결과정상조화가수술조대서비교HIF－1α단백급mRNA표체무명현변화，모형조대서HIF－1α단백급mRNA표체상조，차이무통계학의의；침자조대서HIF－1α단백급mRNA표체고우모형조（0．567±0．058 vs 0．315±0．118；1．593±0．102 vs 1．193±0．259），차이유통계학의의（P＜0．05）。결론뇌결혈후대서뇌조직HIF－1α단백급mRNA표체증강，침자가이상조기표체종이발휘뇌보호작용。
Objective To observe the effect of acupuncture on hypoxia inducible factor -1αand local cerebral blood flow in rats with focal cerebral ischemia.Methods Wistar rats were randomly divided into four groups:normal group, sham-operated group, model group and electroacupuncture( EA) group.Focal cerebral ischemia rat models were developed by middle cerebral artery occlu-sion( MCAO ) with thread embolus.Electroacupuncture was applied to bilateral PC 6 and LI11 after MCAO.The expression of hypoxia inducible factor-1αwas examined in MCAO rats by Western blot and real-time quantitative PCR ( qPCR ) .Results Acupuncture could promote the recovery of neurological function in MCAO rats.Western blot and real-time qPCR showed that acupuncture promoted the hypoxia inducible factor-1αexpression in the ischemic brain tissue with statistical significance( P<0.05) .Conclusion Acupunc-ture promotes hypoxia inducible factor-1αexpression in rats with focal cerebral ischemia.All the results suggest that the increased ex-pression of hypoxia inducible factor-1αin the ischemic brain tissue plays an important role in brain protection.