CAJ | 학술논문

目的：对比分析乙型肝炎（乙肝）前S1、S2抗原和HBV－DNA的相关性，探讨乙肝前S1、S2抗原的临床应用价值。方法随机选取乙肝患者420例，采用ELISA法对前S1、前S2抗原进行检测，采用荧光定量PCR对HBV－DNA进行检测，从血清学标志类型、HBV－DNA载量和HBV感染类型角度对HBV－DNA和前S1、S2抗原检测结果进行分析。结果乙肝大三阳患者乙肝前S1、S2抗原和HBV－DNA阳性率分别为98．48％、95．45％、100．0％；小三阳患者乙肝前S1、S2抗原和HBV－DNA阳性率分别为53．25％、45．45％、46．75％。 HBsAg（＋）HBcAb（＋）患者乙肝前S1、S2抗原和HBV－DNA阳性率分别为53．75％、43．28％、44．78％。在高HBV－DNA载量组中乙肝前S1、S2抗原检出率较高。结论乙肝前S1、S2抗原与HBV－DNA有较好的相关性，可作为反映病毒感染与复制的指标。
목적：대비분석을형간염（을간）전S1、S2항원화HBV－DNA적상관성，탐토을간전S1、S2항원적림상응용개치。방법수궤선취을간환자420례，채용ELISA법대전S1、전S2항원진행검측，채용형광정량PCR대HBV－DNA진행검측，종혈청학표지류형、HBV－DNA재량화HBV감염류형각도대HBV－DNA화전S1、S2항원검측결과진행분석。결과을간대삼양환자을간전S1、S2항원화HBV－DNA양성솔분별위98．48％、95．45％、100．0％；소삼양환자을간전S1、S2항원화HBV－DNA양성솔분별위53．25％、45．45％、46．75％。 HBsAg（＋）HBcAb（＋）환자을간전S1、S2항원화HBV－DNA양성솔분별위53．75％、43．28％、44．78％。재고HBV－DNA재량조중을간전S1、S2항원검출솔교고。결론을간전S1、S2항원여HBV－DNA유교호적상관성，가작위반영병독감염여복제적지표。
Objective To study the relationship between HBV Pre-S1 antigen,HBV Pre-S2 antigen and HBV-DNA and inves-tigate the clinical significance of the hepatitis B virus ( HBV) .Methods The markers Pre-S1 antigen, Pre-S2 antigen were deter-mined by ELISA and HBV-DNA by the fluorescence quantitative PCR in 420 patients with HBV collected from September 2013 to May 2014.HBV, Pre-S1 antigen and Pre-S2 antigen results were analysed in different aspects, such as serum indices, HBV-DNA amounts and HBV-DNA infection types.Results In “big sanyang” patients the positive rates of Pre-S1, S2 antigen and HBV-DNA were 98.48%, 95.45%, 100%,respectively.In“small Sanyang” patients, the positive rates of Pre-S1, S2 antigen and HBV-DNA were 53.25%, 45.45%, 46.75%,respectively.In HBsAg+HBcAb+patients the positive rate of Pre-S1, S2 antigen and HBV-DNA were 53.75%, 43.28%, 44.78%,respectively.And the detection rates of HBV Pre-S1 antigen, HBV Pre-S2 antigen were higher in pa-tients infected with high level of HBV-DNA.Conclusions There is a good correlation between HBV Pre-S1 antigen, HBV Pre-S2 an-tigen and HBV-DNA, which can reflect virus infection and replication.