CAJ | 학술논문

目的：研究银杏叶提取物EGB761对VH－64尤文肉瘤细胞的促凋亡作用。方法 EGB761干预VH－64尤文肉瘤细胞后，CCK－8观察50、100和200 mg／L不同浓度EGB761作用下VH－64尤文肉瘤细胞的增殖状况，流式细胞仪分析用AnnexinV－FITC和PI双染检测不同浓度EGB761对VH－64尤文肉瘤细胞凋亡的影响。结果 EGB761可抑制VH－64尤文肉瘤细胞的增殖，呈浓度和时间依赖性反应；EGB761100 mg／L、200 mg／L干预VH－64尤文肉瘤细胞后，流式细胞仪检测显示细胞凋亡率分别为6．85％±0．36％和14．36％±0．23％，明显高于对照组（ P＜0．01）。结论 EGB761可诱导VH－64尤文肉瘤细胞凋亡。
목적：연구은행협제취물EGB761대VH－64우문육류세포적촉조망작용。방법 EGB761간예VH－64우문육류세포후，CCK－8관찰50、100화200 mg／L불동농도EGB761작용하VH－64우문육류세포적증식상황，류식세포의분석용AnnexinV－FITC화PI쌍염검측불동농도EGB761대VH－64우문육류세포조망적영향。결과 EGB761가억제VH－64우문육류세포적증식，정농도화시간의뢰성반응；EGB761100 mg／L、200 mg／L간예VH－64우문육류세포후，류식세포의검측현시세포조망솔분별위6．85％±0．36％화14．36％±0．23％，명현고우대조조（ P＜0．01）。결론 EGB761가유도VH－64우문육류세포조망。
Objective To explore the contribution of EGB761 to the proliferation and apoptosis of Ewing’ s sarcoma VH-64 cell in vitro.Methods CCK-8 assay was used to measure 50,100 and 200 mg/L which its effects on the proliferation of Ewing’s sar-coma VH-64 cell cultured with different concentrations of allicin for 12 h, 24 h, 48 h, AnnexinV-FITC and PI assay by flow cytometer ( FCM) were applied to detect the apoptosis induced by EGB761.The changes of cell cycle were detected by FCM.Results EGB761 suppressed the proliferation of Ewing’ s sarcoma VH-64 cell in a concentration-and time-dependent manner EGB761 induced cellular apoptosis of the Ewing ’ s sarcoma VH-64 cell in a concentration-dependent manner.With EGB761 100, 200 mg/L intervention Ewing’s sarcoma VH-64 cell, flow cytometer (FCM) test showed that the cell apoptosis rate were 6.85%±0.36% and 14.36%± 0.23%,which were significant higher than that of control group (P<0.01).Conclusions EGB761 significantly suppresses the growth of Ewing’ s sarcoma VH-64 cell, induces the apoptosis of Ewing’ s sarcoma VH-64 cell.Therefore resveratrol may be considered as a possible treatment medicine for Ewing’ s sarcoma.