CAJ | 학술논문

以7个不同黄瓜基因型为材料进行黄瓜未授粉子房的培养研究，结果表明，不同基因型出胚率差异显著，其中密刺型黄瓜北京102出胚率最高，达25.3%，鄂黄3号出胚率最低，为0.7%。以北京102、中农26号和津优1号为试材进一步研究热激诱导、外源激素、植株不同生长阶段对胚状体诱导产量的影响，发现35℃热激处理2 d胚状体产量最高，分化培养基中NAA为0.20 mg·L-1、6-BA为0.8 mg·L-1时最易分化出胚，在植株第21～30节位采收的子房出胚量最高。再生培养基中GA浓度达到0.4～0.8 mg·L-1时，可有效打破再生植株“花打顶”现象从而提高植株再生率。再生植株中二倍体植株比率为70.0%，二倍体植株中DH株比率为84%。
이7개불동황과기인형위재료진행황과미수분자방적배양연구，결과표명，불동기인형출배솔차이현저，기중밀자형황과북경102출배솔최고，체25.3%，악황3호출배솔최저，위0.7%。이북경102、중농26호화진우1호위시재진일보연구열격유도、외원격소、식주불동생장계단대배상체유도산량적영향，발현35℃열격처리2 d배상체산량최고，분화배양기중NAA위0.20 mg·L-1、6-BA위0.8 mg·L-1시최역분화출배，재식주제21～30절위채수적자방출배량최고。재생배양기중GA농도체도0.4～0.8 mg·L-1시，가유효타파재생식주“화타정”현상종이제고식주재생솔。재생식주중이배체식주비솔위70.0%，이배체식주중DH주비솔위84%。
Un-pollinated ovary culture was applied in 7 different cucumber(Cucumis sativusL.) varieties. The embryo yield was significantly different among different genotypes.Among them, the highest embryo formation rate was‘Beijing 102’, reaching 25.3%, the lowest embryo formation rate was‘Erhuang No.3’, reaching 0.7%. Taking‘Beijing 102’,‘Zhongnong No.26’ and‘Jinyou No.1’as experimental material, further studies were conducted about the effects of thermal treatment, exogenous hormones and different growth stage of plant on embryo induction yield.The results showed that a thermal shock at 35 ℃ for 2 days could gain the highest embryo yield, and the differential medium with 0.20 mg·L-1NAA and 0.8 mg·L-16-BA was optimal for embryo differentiation.The highest embryo yield was harvested with ovary selected at 21-30 nodes of the plant stem. When the GA concentration in regeneration medium reached 0.4-0.8 mg·L-1,the ‘blunt with blossom’ phenomenon of regenerated plant could be effectively vanished.Thus, the plant regeneration rate was increased. The ratio of diploid plants in regenerated plants was 70%,and the DH plant ratio in diploid plants was 84%.