CAJ | 학술논문

采用HPLC法，对11个结球甘蓝叶片硫代葡萄糖苷（硫苷）组分及含量进行测定。结果表明：结球甘蓝叶片中共含有9种硫苷，分别为3-甲基硫氧烯丙基硫苷（IBE）、2-羟基-3-丁烯基硫苷（PRO）、2-丙烯基硫苷（SIN）、4-甲基硫氧丁基硫苷（RAA）和3-丁烯基硫苷（NAP）等5种脂肪族硫苷，4-羟基吲哚基-3-甲基硫苷（4OH）、3-甲基吲哚基硫苷（GBC）、4-甲氧基吲哚基-3-甲基硫苷（4ME）和1-甲氧基吲哚基-3-甲基硫苷（NEO）等4种吲哚族硫苷；IBE、SIN和GBC是结球甘蓝叶片中硫苷的主要组分，三者总量占总硫苷含量的63.60%～91.06%。结球甘蓝叶片总硫苷含量在3.59～19.70μmol·g-1（DW）之间，总脂肪族硫苷含量在2.74～15.15μmol·g-1（DW）之间，总吲哚族硫苷含量在0.81～7.19μmol·g-1（DW）之间。
채용HPLC법，대11개결구감람협편류대포도당감（류감）조분급함량진행측정。결과표명：결구감람협편중공함유9충류감，분별위3-갑기류양희병기류감（IBE）、2-간기-3-정희기류감（PRO）、2-병희기류감（SIN）、4-갑기류양정기류감（RAA）화3-정희기류감（NAP）등5충지방족류감，4-간기신타기-3-갑기류감（4OH）、3-갑기신타기류감（GBC）、4-갑양기신타기-3-갑기류감（4ME）화1-갑양기신타기-3-갑기류감（NEO）등4충신타족류감；IBE、SIN화GBC시결구감람협편중류감적주요조분，삼자총량점총류감함량적63.60%～91.06%。결구감람협편총류감함량재3.59～19.70μmol·g-1（DW）지간，총지방족류감함량재2.74～15.15μmol·g-1（DW）지간，총신타족류감함량재0.81～7.19μmol·g-1（DW）지간。
Taking 11 cabbage(Brassica oleracea L. var.capitata L.)varieties as experimental material,their glucosinolate profiles and contents in leaves were evaluated by HPLC method. The results indicated that 9 types of glucosinolate were existed in cabbage leaves,including 5 aliphatic glucosinolates (Glucoiberin,Sinigrin,Progoitrin,Glucoraphanin and Gluconapin),and 4 indolic glucosinolates(4-Hydroxy glucobrassicin,Glucobrassicin,4-Methoxy glucobrassicin and Neoglucobrassicin). Glucoiberin,Sinigrin and Glucobrassicin were the main glucosinolate profiles in cabbage leaves. The 3 glucosinolates ranged from 63.60%-91.06% of the total glucosinolate contents. The contets of total glucosinolate,total aliphatic glucosinolate and total indolic glucosinolate ranged 3.59-19.70 μmol·g-1(DW),2.74-15.15μmol·g-1(DW),and 0.81-7.19μmol·g-1 (DW),respectively.