福建畜牧兽医
福建畜牧獸醫
복건축목수의
FUJIAN JOURNAL OF ANIMAL HUSBANDRY AND VETERINARY
2015年
3期
1-2
,共2页
薄荷脑%薄荷素油%气相色谱法%无水乙醇
薄荷腦%薄荷素油%氣相色譜法%無水乙醇
박하뇌%박하소유%기상색보법%무수을순
Menthol%Peppermint oil%Gas chromatography%Dehydrated alcohol
目的：建立气相色谱测定混合型饲料添加剂薄荷素油中薄荷脑含量的方法。方法样品采用乙醇提取，以毛细管色谱柱分离，采用氢火焰离子化检测器检测。结果薄荷脑在0.0356~1.780 mg/mL 范围内具有良好的线性关系，线性方程为 Y=139286×X-3452，相关系数R为0.9998，方法的定量限（LOQ）为0.1 g/kg。样品添加回收试验的平均回收率在98.2.8%~99.2%，相对标准偏差（RSD）为1.06%~1.96%（n=6）。结论本方法简便、快速，结果准确、可靠，适用于混合型饲料添加剂薄荷素油中薄荷脑含量的测定。
目的：建立氣相色譜測定混閤型飼料添加劑薄荷素油中薄荷腦含量的方法。方法樣品採用乙醇提取，以毛細管色譜柱分離，採用氫火燄離子化檢測器檢測。結果薄荷腦在0.0356~1.780 mg/mL 範圍內具有良好的線性關繫，線性方程為 Y=139286×X-3452，相關繫數R為0.9998，方法的定量限（LOQ）為0.1 g/kg。樣品添加迴收試驗的平均迴收率在98.2.8%~99.2%，相對標準偏差（RSD）為1.06%~1.96%（n=6）。結論本方法簡便、快速，結果準確、可靠，適用于混閤型飼料添加劑薄荷素油中薄荷腦含量的測定。
목적：건립기상색보측정혼합형사료첨가제박하소유중박하뇌함량적방법。방법양품채용을순제취，이모세관색보주분리，채용경화염리자화검측기검측。결과박하뇌재0.0356~1.780 mg/mL 범위내구유량호적선성관계，선성방정위 Y=139286×X-3452，상관계수R위0.9998，방법적정량한（LOQ）위0.1 g/kg。양품첨가회수시험적평균회수솔재98.2.8%~99.2%，상대표준편차（RSD）위1.06%~1.96%（n=6）。결론본방법간편、쾌속，결과준학、가고，괄용우혼합형사료첨가제박하소유중박하뇌함량적측정。
Objective A method for the determination of menthol in feed additive blender Peppermint oil by gas chromatography was established. Methods The sample was firstly extracted by alcohol, and then was separated by capillary chromatogram column, and fi-nally was determinate by flame ionization detector. Results The linear relation of menthol in 0.0356 to 1.780 mg/mL showed good lin-earity (R=0.9998). The linear equation was Y=139 286 ×X-3 452. The limit of quantization (LOQ) was 0.1 g/kg. The average recovery rate of recovery test was 98. 8%to 99.2%. The relative standard deviation (RSD)was 1.06%to 1.96%(n=6). Conclusion This method was simple, rapid and accurate. It can be used as a method for the determination of menthol in feed additive blender Peppermint Oil.
