CAJ | 학술논문

目的：观察薯蓣丸对慢性肾衰竭（ CRF）大鼠红细胞计数（ RBC）、血红蛋白（ Hgb）含量及肾组织转化生长因子β1（TGF －β1）表达的影响。方法将70只雄性 SD 大鼠分为假手术组10只及手术组60只。手术组均采用5／6肾切除方法制备 CRF 模型，再随机分为3组即著蓣丸组（13只）、包醛氧淀粉组（14只）及模型组（13只）。薯蓣丸组以1．8 g／（kg? d）、包醛氧淀粉组以2 g／（kg? d）相应药液定时灌胃，假手术组及模型组每日同时间以等容积0．9％氯化钠注射液灌胃。6周后测大鼠血清 RBC、Hgb 含量，制作肾组织苏木精—伊红（HE）染色切片观察病变程度，免疫组化法测大鼠肾组织 TGF －β1的表达情况。结果假手术组、薯蓣丸组及包醛氧淀粉组 RBC、Hgb 含量均高于模型组（P ＜0．05，P ＜0．01），薯蓣丸组 RBC、Hgb 含量高于包醛氧淀粉组（P ＜0．05）。 HE 切片显示模型组病变程度明显，薯蓣丸组及包醛氧淀粉组病变程度较模型组均有所好转，薯蓣丸组病变轻于包醛氧淀粉组。假手术组、薯蓣丸组及包醛氧淀粉组肾组织 TGF －β1阳性表达量低于模型组（P ＜0．05，P ＜0．01），且薯蓣丸组低于包醛氧淀粉组（P ＜0．05）。结论薯蓣丸可升高CRF 大鼠 RBC 及 Hgb 含量，降低大鼠肾组织 TGF －β1水平，减轻肾脏病变。
목적：관찰서여환대만성신쇠갈（ CRF）대서홍세포계수（ RBC）、혈홍단백（ Hgb）함량급신조직전화생장인자β1（TGF －β1）표체적영향。방법장70지웅성 SD 대서분위가수술조10지급수술조60지。수술조균채용5／6신절제방법제비 CRF 모형，재수궤분위3조즉저여환조（13지）、포철양정분조（14지）급모형조（13지）。서여환조이1．8 g／（kg? d）、포철양정분조이2 g／（kg? d）상응약액정시관위，가수술조급모형조매일동시간이등용적0．9％록화납주사액관위。6주후측대서혈청 RBC、Hgb 함량，제작신조직소목정—이홍（HE）염색절편관찰병변정도，면역조화법측대서신조직 TGF －β1적표체정황。결과가수술조、서여환조급포철양정분조 RBC、Hgb 함량균고우모형조（P ＜0．05，P ＜0．01），서여환조 RBC、Hgb 함량고우포철양정분조（P ＜0．05）。 HE 절편현시모형조병변정도명현，서여환조급포철양정분조병변정도교모형조균유소호전，서여환조병변경우포철양정분조。가수술조、서여환조급포철양정분조신조직 TGF －β1양성표체량저우모형조（P ＜0．05，P ＜0．01），차서여환조저우포철양정분조（P ＜0．05）。결론서여환가승고CRF 대서 RBC 급 Hgb 함량，강저대서신조직 TGF －β1수평，감경신장병변。
Objective To observe the effect of Shuyu pill on the contents of red blood cells (RBC),hemo-globin(Hgb) and expression of nephridial tissue transforming growth factor beta 1(TGF -β1 ) in rats with chronic re-nal failure(CRF).Methods 70 male SD rats were divided into sham operation group ,model group,Shuyu pill group and oxyamyli tectus aldehydum group .CRF model in model group,Shuyu pill group and oxyamyli tectus aldehydum group were induced by 5 /6 nephrectom.Rats in Shuyu group and oxyamyli tectus aldehydum group received 1.8 g/(kg? d) and 2 g/(kg? d) of Shuyu pill via gastrogavage respectively .The other rats received isasteric 0.9% sodi-um chloride injection by gastrogavage .After 6 weeks of experiment,the levels of RBC and Hgb were detected ,patho-logical change of kidney -tissue by HE staining were observed,and expression of TGF -β1 were measured by immu-nohistochemistry.Results The contents of RBC and Hgb in sham operation group ,Shuyu pill group and oxyamyli tectus aldehydum group were higher than those in model group ( P <0.05, P <0.01).The contents of RBC and Hgb in Shuyu pill group were higher than those in oxyamyli tectus aldehydum group ( P <0.05).Pathological change of kidney -tissue was obvious in model group .Lesion extent in Shuyu pill group and oxyamyli tectus aldehy -dum group was less than that in model group .Positive expression quantity of TGF -β1 of nephridial tissue in sham operation group, Shuyu pill group and oxyamyli tectus aldehydum group were lower than those in model group ( P<0.05, P <0.01).Pathological change of kidney -tissue and positive expression of TGF -β1 in Shuyu pill group were lower than those in oxyamyli tectus aldehydum group ( P <0.05).Conclusion Shuyu pill can enhance RBC and Hgb in rats with CRF,and decrease expression of TGF -β1 in kidney -tissue.