CAJ | 학술논문

目的：研究缺氧诱导因子-1α在胆囊癌侵袭转移中的作用，并探讨 HIF-1α在判断胆囊癌治疗效果及预测胆囊癌复发转移方面的价值。方法：采用慢病毒对胆囊癌细胞系（GBC-SD）进行转染，干扰该细胞系中HIF-1α的表达，得到 HIF-1α表达不受干扰的胆囊癌细胞系（GBC-SD-NC）和 HIF-1α表达受干扰的胆囊癌细胞系 GBC-SD-HIF。通过将两组细胞系裸鼠鼠尾静脉注射建立胆囊癌血行转移模型，检测两组细胞在小鼠肺部形成转移瘤的能力。结果：GBC-SD-NC 组裸鼠肺部转移瘤数量、大小都比 GBC-SD-HIF 组高，GBC-SD-NC 组转移瘤的平均数量显著多于 GBC-SD-HIF 组。结论：干扰胆囊癌细胞中 HIF-1α的表达，可以显著减低胆囊癌细胞系GBC-SD 的血行肺转移能力。说明缺氧诱导因子-1α在胆囊癌侵袭转移中存在独立影响。
목적：연구결양유도인자-1α재담낭암침습전이중적작용，병탐토 HIF-1α재판단담낭암치료효과급예측담낭암복발전이방면적개치。방법：채용만병독대담낭암세포계（GBC-SD）진행전염，간우해세포계중HIF-1α적표체，득도 HIF-1α표체불수간우적담낭암세포계（GBC-SD-NC）화 HIF-1α표체수간우적담낭암세포계 GBC-SD-HIF。통과장량조세포계라서서미정맥주사건립담낭암혈행전이모형，검측량조세포재소서폐부형성전이류적능력。결과：GBC-SD-NC 조라서폐부전이류수량、대소도비 GBC-SD-HIF 조고，GBC-SD-NC 조전이류적평균수량현저다우 GBC-SD-HIF 조。결론：간우담낭암세포중 HIF-1α적표체，가이현저감저담낭암세포계GBC-SD 적혈행폐전이능력。설명결양유도인자-1α재담낭암침습전이중존재독립영향。
Objective To study the effects of HIF-1αon the metastasis of gallbladder neoplasm, and discusses the value of HIF-1α on gallbladder neoplasm treatment. Methods Lentivirus infections were carried on to obtain the GBC-SD-NC whose expression of HIF-1α was normal,and GBC-SD-HIF that expression of HIF-1α was interfered. HIF-1α expression was in-terfered through vena caudalis in nude mice exenografts. HIF-1α expression effect was demonstrated on the metastasis ability of gallbladder neoplasm cells. Results GBC-SD-NC group whose number of metastasis and volume of metastasis were re-markably superior to the GBC-SD-HIF group. In In GBC-SD-NC group, the average number of metastasis foci permouse was marked more than GBC-SD-HIF group. Conclusion The interference of HIF-1α expression could lead to the decline of the metastasis ability of gallbladder neoplasm cells. The Results showed that HIF-1α could produce effects in on the metastasis of gallbladder neoplasm.