实用医药杂志
實用醫藥雜誌
실용의약잡지
Practical Journal of Medicine & Pharmacy
2015年
6期
535-537
,共3页
双醋瑞因%黄韧带肥厚%病理学%免疫组织化学
雙醋瑞因%黃韌帶肥厚%病理學%免疫組織化學
쌍작서인%황인대비후%병이학%면역조직화학
Diacerein%Ligament flavum hypertrophy%Pathology%Immunohistochemistry
目的：观察双醋瑞因对黄韧带肥厚的作用机制，探讨应用双醋瑞因预防和治疗黄韧带肥厚的效果。方法对黄韧带肥厚的大白兔分别应用双醋瑞因作药物组（45只），与生理盐水灌胃做模型组（45只），正常大白兔45只作为正常对照组；于第4、8和12周，随机处死各组动物至少15只，取黄韧带，采用免疫组化的方法观察三组免疫组化切片中IL－1、IL－6及TNF－α的表达情况。结果模型组与药物组中黄韧带细胞质或细胞核中IL－1、IL－6、TNF－α的表达明显高于正常组（P＜0．05）。药物组的IL－1、IL－6、TNF－α表达阳性率显著低于模型组（P＜0．05）。结论双醋瑞因对局部组织的炎症因子起到了改善作用；通过双醋瑞因的药物干预，肥厚黄韧带中胶原纤维增生明显受到抑制，从而改善了肥厚黄韧带中的组织结构，可使之恢复至正常状态或接近正常状态。
目的：觀察雙醋瑞因對黃韌帶肥厚的作用機製，探討應用雙醋瑞因預防和治療黃韌帶肥厚的效果。方法對黃韌帶肥厚的大白兔分彆應用雙醋瑞因作藥物組（45隻），與生理鹽水灌胃做模型組（45隻），正常大白兔45隻作為正常對照組；于第4、8和12週，隨機處死各組動物至少15隻，取黃韌帶，採用免疫組化的方法觀察三組免疫組化切片中IL－1、IL－6及TNF－α的錶達情況。結果模型組與藥物組中黃韌帶細胞質或細胞覈中IL－1、IL－6、TNF－α的錶達明顯高于正常組（P＜0．05）。藥物組的IL－1、IL－6、TNF－α錶達暘性率顯著低于模型組（P＜0．05）。結論雙醋瑞因對跼部組織的炎癥因子起到瞭改善作用；通過雙醋瑞因的藥物榦預，肥厚黃韌帶中膠原纖維增生明顯受到抑製，從而改善瞭肥厚黃韌帶中的組織結構，可使之恢複至正常狀態或接近正常狀態。
목적：관찰쌍작서인대황인대비후적작용궤제，탐토응용쌍작서인예방화치료황인대비후적효과。방법대황인대비후적대백토분별응용쌍작서인작약물조（45지），여생리염수관위주모형조（45지），정상대백토45지작위정상대조조；우제4、8화12주，수궤처사각조동물지소15지，취황인대，채용면역조화적방법관찰삼조면역조화절편중IL－1、IL－6급TNF－α적표체정황。결과모형조여약물조중황인대세포질혹세포핵중IL－1、IL－6、TNF－α적표체명현고우정상조（P＜0．05）。약물조적IL－1、IL－6、TNF－α표체양성솔현저저우모형조（P＜0．05）。결론쌍작서인대국부조직적염증인자기도료개선작용；통과쌍작서인적약물간예，비후황인대중효원섬유증생명현수도억제，종이개선료비후황인대중적조직결구，가사지회복지정상상태혹접근정상상태。
Objective To observe the action mechanism of diacerein on hypertrophy of ligamentum flavum,and explore prevention and treatment of diacerein for ligamentum flavum hypertrophy. Methods The flavum ligament hypertrophy of rabbits were respectively given diacerein (as drug group,n=45),normal saline viastomach(n=45,as model group),and 45 normal rabbits as normal control group;at the fourth,eighth and twelfth weeks,each group were randomly sacrificed animal at least 15,the flavum ligament was taken out,the three groups were detected in their IL -1,IL -6 and TNF -alpha in slice mining with the methods of immunohistochemistry. Results The expression of flavum ligament cytoplasm or nucleus IL-1,IL-6,TNF-alpha in model group and drug group was significantly higher than that in normal group,there was statistical difference(P<0.05). The positive expression of IL-1,IL-6,TNF-alpha rate in drug group was significantly lower than that in model group,there was statistical difference(P<0.05). Conclusion Diacerein plays a positive improvement on local tissue inflammation factor,by diacerin intervention,in ligamentum flavum hypertrophy collagen fiber hyperplasia is obviously inhibited,thereby improving the organization structure of the ligamentum flavum hypertrophy,so restored to normal state or close to normal state.
