川北医学院学报
川北醫學院學報
천북의학원학보
JOURNAL OF NORTH SICHUAN MEDICAL COLLEGE
2015年
3期
314-317,339
,共5页
乙型肝炎病毒%病毒载量%细胞免疫%T细胞亚群%自然杀伤细胞
乙型肝炎病毒%病毒載量%細胞免疫%T細胞亞群%自然殺傷細胞
을형간염병독%병독재량%세포면역%T세포아군%자연살상세포
Hepatitis B virus%Viral load%Cellular immunity%T lymphocyte subsets%Natural killer cell
目的::探讨慢性乙型肝炎病毒( HBV)携带者的HBV-DNA载量与细胞免疫功能的关系。方法:选择本院诊治的HBV携带者为观察组(90例),根据荧光定量PCR法测定HBV-DNA 含量分为阴性组(14例)、低拷贝组(49例)、高拷贝组(27例),同期体检合格的健康人群为对照组(90例),观察各组受试者外周血 T 细胞亚群 CD3+、CD4+、CD8+百分比和CD4+/CD8+值,外周血B淋巴细胞、自然杀伤细胞( NK)、活化T细胞比例,及CD4+T淋巴细胞和CD8+T淋巴细胞表面的PD-1阳性率和PD-1平均荧光强度。结果:观察组患者CD3+、CD4+百分比、CD4+/CD8+值、NK细胞比例均低于对照组,差异有统计学意义(P<0.01),CD8+百分比、CD4+T淋巴细胞和CD8+T淋巴细胞表面的PD-1阳性率和PD-1平均荧光强度高于对照组,差异有统计学意义(P<0.01)。随着HBV-DNA病毒拷贝数的增加,CD3+、CD4+百分比、CD4+/CD8+值、NK细胞比例降低(P<0.05或P<0.01),CD8+百分比、CD4+T淋巴细胞和CD8+T淋巴细胞表面的PD-1阳性率及PD-1平均荧光强度上升(P<0.05或P<0.01)。结论:慢性乙型肝炎病毒携带者的HBV-DNA病毒载量与外周紊乱的T 淋巴细胞之间存在密切联系,临床可动态监测HBV-DNA病毒载量与T 淋巴细胞的变化,以指导临床治疗。
目的::探討慢性乙型肝炎病毒( HBV)攜帶者的HBV-DNA載量與細胞免疫功能的關繫。方法:選擇本院診治的HBV攜帶者為觀察組(90例),根據熒光定量PCR法測定HBV-DNA 含量分為陰性組(14例)、低拷貝組(49例)、高拷貝組(27例),同期體檢閤格的健康人群為對照組(90例),觀察各組受試者外週血 T 細胞亞群 CD3+、CD4+、CD8+百分比和CD4+/CD8+值,外週血B淋巴細胞、自然殺傷細胞( NK)、活化T細胞比例,及CD4+T淋巴細胞和CD8+T淋巴細胞錶麵的PD-1暘性率和PD-1平均熒光彊度。結果:觀察組患者CD3+、CD4+百分比、CD4+/CD8+值、NK細胞比例均低于對照組,差異有統計學意義(P<0.01),CD8+百分比、CD4+T淋巴細胞和CD8+T淋巴細胞錶麵的PD-1暘性率和PD-1平均熒光彊度高于對照組,差異有統計學意義(P<0.01)。隨著HBV-DNA病毒拷貝數的增加,CD3+、CD4+百分比、CD4+/CD8+值、NK細胞比例降低(P<0.05或P<0.01),CD8+百分比、CD4+T淋巴細胞和CD8+T淋巴細胞錶麵的PD-1暘性率及PD-1平均熒光彊度上升(P<0.05或P<0.01)。結論:慢性乙型肝炎病毒攜帶者的HBV-DNA病毒載量與外週紊亂的T 淋巴細胞之間存在密切聯繫,臨床可動態鑑測HBV-DNA病毒載量與T 淋巴細胞的變化,以指導臨床治療。
목적::탐토만성을형간염병독( HBV)휴대자적HBV-DNA재량여세포면역공능적관계。방법:선택본원진치적HBV휴대자위관찰조(90례),근거형광정량PCR법측정HBV-DNA 함량분위음성조(14례)、저고패조(49례)、고고패조(27례),동기체검합격적건강인군위대조조(90례),관찰각조수시자외주혈 T 세포아군 CD3+、CD4+、CD8+백분비화CD4+/CD8+치,외주혈B림파세포、자연살상세포( NK)、활화T세포비례,급CD4+T림파세포화CD8+T림파세포표면적PD-1양성솔화PD-1평균형광강도。결과:관찰조환자CD3+、CD4+백분비、CD4+/CD8+치、NK세포비례균저우대조조,차이유통계학의의(P<0.01),CD8+백분비、CD4+T림파세포화CD8+T림파세포표면적PD-1양성솔화PD-1평균형광강도고우대조조,차이유통계학의의(P<0.01)。수착HBV-DNA병독고패수적증가,CD3+、CD4+백분비、CD4+/CD8+치、NK세포비례강저(P<0.05혹P<0.01),CD8+백분비、CD4+T림파세포화CD8+T림파세포표면적PD-1양성솔급PD-1평균형광강도상승(P<0.05혹P<0.01)。결론:만성을형간염병독휴대자적HBV-DNA병독재량여외주문란적T 림파세포지간존재밀절련계,림상가동태감측HBV-DNA병독재량여T 림파세포적변화,이지도림상치료。
Objective:To investigate the relationship between hepatitis B virus ( HBV) DNA loads and cellular immunity in chronic HBV carriers. Methods:A total of 90 chronic HBV carriers as observation group assigned to negative group (n=14),low copy group (n=49) and high copy group (n=27) according to their HBV-DNA load tested by fluorescence quantitative PCR. Another 90 health participants were enrolled as control group. The percentage of CD3 +,CD4 + and CD8 + T lymphocyte,CD4 + /CD8 +,proportion of B lymphocyte and natural killer (NK) cell in peripheral blood,positive rate and mean fluorescence intensity of PD-1 on surface of CD4 + and CD8 + T lymphocyte were detected in all groups,respectively. Results:the percentage of CD3 + and CD4 + T lymphocyte, CD4 + /CD8 + and proportion of NK cell were significantly lower in observation group than those in control group (P<0. 01),and the percentage of CD8 + lymphocyte,positive rate and mean fluorescence intensity of PD-1 were significantly higher in observation group than those in control group (P<0. 01). The percentage of CD3 + and CD4 + T lymphocyte,CD4 + /CD8 + and proportion of NK cell were increased (P<0. 05,P<0. 01),and the percentage of CD8 + lymphocyte,positive rate and mean fluorescence intensity of PD-1 were decreased (P<0. 05,P<0. 01),with an increase in copy of HBV-DNA increased. Conclusion:There is a potential correlation between HBV-DNA loads and cellular immunity in chronic HBV carriers. Dynamically monitoring on HBV-DNA loads and T lymphocyte can offer valuable reference to clinical treatment.