CAJ | 학술논문

为了克隆草地藏系绵羊生长激素释放激素基因,采用Trizol法从草地藏系绵羊下丘脑组织中提取总RNA,用反转录-聚合酶链式反应( RT-PCR)进行cDNA扩增并克隆测序,获得长度为207 bp的促生长激素释放激素基因( GHRH)的部分cDNA序列。结果表明获得的草地藏系绵羊GHRH部分cDNA序列与GenBank中注册的绵羊GHRH基因编码起始位置(86位)到292位区域高度同源,仅有1个碱基的差异,该cDNA序列编码69个氨基酸残基,其内含有信号肽序列,该氨基酸序列的31~57位具有典型胰高血糖素类似激素特征的GLUCA结构域。
위료극륭초지장계면양생장격소석방격소기인,채용Trizol법종초지장계면양하구뇌조직중제취총RNA,용반전록-취합매련식반응( RT-PCR)진행cDNA확증병극륭측서,획득장도위207 bp적촉생장격소석방격소기인( GHRH)적부분cDNA서렬。결과표명획득적초지장계면양GHRH부분cDNA서렬여GenBank중주책적면양GHRH기인편마기시위치(86위)도292위구역고도동원,부유1개감기적차이,해cDNA서렬편마69개안기산잔기,기내함유신호태서렬,해안기산서렬적31~57위구유전형이고혈당소유사격소특정적GLUCA결구역。
Total RNA was extracted from the hypothalamus tissues of Tibetan sheep using Trizol method and cDNA was amplified by reverse transcription polymerase chain reaction ( RT-PCR) . The cDNA of Tibetan sheep GHRH gene was cloned from the amplified PCR product and sequenced. The cDNA was 207 bp in length and showed 99% homology with that of com-mon sheep registered in GenBank from the starting position (86) to 292 bp. The cDNA sequence encodes 69 amino acid resi-dues and contains a signal peptide sequence which has a GLUCA domain characterizing glucagon-like hormone at amino acid 31 to 57.