CAJ | 학술논문

为探讨生长分化因子9(Growth differentiation factor 9, GDF9)和卵泡抑素(Follistatin,FST)如何影响猪卵母细胞成熟及胚胎早期发育能力,在猪卵母细胞体外成熟( IVM)培养过程中添加不同浓度的GDF9和抗GDF9抗体,或同时添加GDF9和FST或抗FST抗体,测定猪卵母细胞的细胞核成熟率、卵裂率、囊胚率及囊胚总细胞数。结果显示,添加GDF9显著提高孤雌胚囊胚率,抗GDF9抗体则显著抑制卵裂率和囊胚率。添加FST显著降低孤雌胚囊胚率和囊胚总细胞数,添加抗FST抗体显著提高了囊胚率。共同添加GDF9与抗FST抗体则使囊胚率达到最高。表明猪卵母细胞IVM期添加GDF9能一定程度上提高卵母细胞及胚胎的发育能力,共同添加GDF9和抗FST抗体对胚胎发育的促进作用具有加性效应。
위탐토생장분화인자9(Growth differentiation factor 9, GDF9)화란포억소(Follistatin,FST)여하영향저란모세포성숙급배태조기발육능력,재저란모세포체외성숙( IVM)배양과정중첨가불동농도적GDF9화항GDF9항체,혹동시첨가GDF9화FST혹항FST항체,측정저란모세포적세포핵성숙솔、란렬솔、낭배솔급낭배총세포수。결과현시,첨가GDF9현저제고고자배낭배솔,항GDF9항체칙현저억제란렬솔화낭배솔。첨가FST현저강저고자배낭배솔화낭배총세포수,첨가항FST항체현저제고료낭배솔。공동첨가GDF9여항FST항체칙사낭배솔체도최고。표명저란모세포IVM기첨가GDF9능일정정도상제고란모세포급배태적발육능력,공동첨가GDF9화항FST항체대배태발육적촉진작용구유가성효응。
To investigate the effects of growth differentiation factor 9 (GDF9) and follistatin (FST) on porcine oocyte maturation and early embryo developmental competence,different concentrations of exogenous GDF9, anti-GDF9 antibody, FST and anti-FST antibody were added into in vitro maturation medium,and the nuclear maturation, cleavage, blastocyst rate and blastocyst cell number were detected. GDF9 significantly improved blastocyst rate. Anti-GDF9 antibody significantly inhibited cleavage and blastocyst rates. FST addition reduced parthenogenic embryo blastocyst rate and the total cell number of blastocyst. Maturation medium containing anti-FST antibody significantly improved blastocyst rates. Maturation medium containing GDF9 and anti-FST antibody together drove parthenogenic embryo blastocyst rate to the highest level. The results suggested GDF9 addition in maturation medium could improve oocyte maturation and early embryo developmental competence. GDF9 and anti-FST antibody in maturation medium could synergistically improve oocyte developmental competence.