湖南师范大学学报（医学版）
湖南師範大學學報（醫學版）
호남사범대학학보（의학판）
JOURNAL OF HUNAN NORMAL UNIVERSITY(MEDICAL SCIENCE)
2015年
2期
24-27
,共4页
肝细胞癌%富亮氨酸重复蛋白 1%侵袭转移
肝細胞癌%富亮氨痠重複蛋白 1%侵襲轉移
간세포암%부량안산중복단백 1%침습전이
hepatocellular carcinoma%leucine-rich repeat protein 1%invasion and metastasis
目的：研究富亮氨酸重复1（leucine-rich-alpha-2-glycoprotein 1，LRG1）在人肝细胞癌（hepatocellular carcinoma, HCC）中的表达情况，探讨其与 HCC 的相关性。方法：选取6个肝癌细胞系及30对连续的新鲜冰冻HCC 组织及其相应的癌旁非瘤肝组织标本，采取 qRT-PCR 检测 LRG1mRNA 的表达；Western blot 检测 LRG1的蛋白表达。结果：肝癌细胞系中 LRG1的 mRNA、蛋白的表达水平明显高于正常肝细胞系 LO2，LRG1的表达量与肝癌细胞系的侵袭转移能力正相关，新鲜冰冻组织中 LRG1的 mRNA、蛋白的表达水平明显高于相应的癌旁非瘤肝组织。结论：LRG1的高表达与 HCC 的恶性程度密切相关，对其研究可用于研发新的靶向药物。
目的：研究富亮氨痠重複1（leucine-rich-alpha-2-glycoprotein 1，LRG1）在人肝細胞癌（hepatocellular carcinoma, HCC）中的錶達情況，探討其與 HCC 的相關性。方法：選取6箇肝癌細胞繫及30對連續的新鮮冰凍HCC 組織及其相應的癌徬非瘤肝組織標本，採取 qRT-PCR 檢測 LRG1mRNA 的錶達；Western blot 檢測 LRG1的蛋白錶達。結果：肝癌細胞繫中 LRG1的 mRNA、蛋白的錶達水平明顯高于正常肝細胞繫 LO2，LRG1的錶達量與肝癌細胞繫的侵襲轉移能力正相關，新鮮冰凍組織中 LRG1的 mRNA、蛋白的錶達水平明顯高于相應的癌徬非瘤肝組織。結論：LRG1的高錶達與 HCC 的噁性程度密切相關，對其研究可用于研髮新的靶嚮藥物。
목적：연구부량안산중복1（leucine-rich-alpha-2-glycoprotein 1，LRG1）재인간세포암（hepatocellular carcinoma, HCC）중적표체정황，탐토기여 HCC 적상관성。방법：선취6개간암세포계급30대련속적신선빙동HCC 조직급기상응적암방비류간조직표본，채취 qRT-PCR 검측 LRG1mRNA 적표체；Western blot 검측 LRG1적단백표체。결과：간암세포계중 LRG1적 mRNA、단백적표체수평명현고우정상간세포계 LO2，LRG1적표체량여간암세포계적침습전이능력정상관，신선빙동조직중 LRG1적 mRNA、단백적표체수평명현고우상응적암방비류간조직。결론：LRG1적고표체여 HCC 적악성정도밀절상관，대기연구가용우연발신적파향약물。
Objective To investigate the expression of leucine-rich-alpha-2-glycoprotein 1(LRG1 ) in hepatocellular car-cinoma (HCC) Cell lines and tissue, and to explore its relationship with HCC progression. Methods qRT-PCR and Western blot analysis were used to detect LRG1 mRNA and protein expression respectively in 6 hepatocellular carcinoma cell lines, 30 paired HCC and adjacent normal liver tissues. Results The levels of LRG1mRNA and protein expression hepatocellular car-cinoma cell lines mRNA, protein levels LRG1 significantly higher than normal liver cells L02, the of invasion and metastasis of HCC cell lines positive correlation with LRG1 expression levels. The levels of LRG1 mRNA and protein expression in HCC were significantly higher than those in adjacent liver tissues. Conclusion LRG1 overexpression has a significant correlation with the progression of HCC, and it would designing new targeted drugs.
