CAJ | 학술논문

利用相关序列扩增多态性( SRAP)和群体分离分析法( BSA)对棉花耐盐材料×敏盐材料组合的F2群体及26个耐、敏品种(系)进行分析和鉴定，筛选与棉花耐盐相关的分子标记。在88对正反引物组合中，筛选出在2个亲本间具有多态性的引物6对，经F2群体分析，发现1对引物能够在耐盐单株中扩增出350 bp的特异片段，而在敏盐单株中没有。品种鉴定显示，耐盐材料均能扩增出该片段，而敏盐材料没有该片段扩增。推测该片段是与棉花耐盐性紧密连锁的分子标记。
이용상관서렬확증다태성( SRAP)화군체분리분석법( BSA)대면화내염재료×민염재료조합적F2군체급26개내、민품충(계)진행분석화감정，사선여면화내염상관적분자표기。재88대정반인물조합중，사선출재2개친본간구유다태성적인물6대，경F2군체분석，발현1대인물능구재내염단주중확증출350 bp적특이편단，이재민염단주중몰유。품충감정현시，내염재료균능확증출해편단，이민염재료몰유해편단확증。추측해편단시여면화내염성긴밀련쇄적분자표기。
Twenty-six salt tolerant and sensitive materials, and a F2 population derived from the cross between a salt tolerant material and a salt sensitive material were analyzed by sequence-related amplified polymorphism ( SRAP ) marker and bulked segrgant analysis( BSA) method to screen markers associated with salt tolerance of cotton. Six pairs of 88 prim-er combinations were detected polymorphic in both parents, among which, one pair of primer amplified a 350-bp fragment in the tolerant individuals of F2 population, but no band in the susceptible individuals. The fragment were identified exclu-sively in 22 salt tolerant varieties. The results suggested that this marker might be closely linked to salt tolerance in cotton.