CAJ | 학술논문

目的：建立鉴定A型肉毒毒素的ELISA鉴别试验方法以替代传统的动物试验法。方法采用现代免疫学技术，制备马源性和兔源性抗A型肉毒毒素多克隆抗体，建立了双抗体夹心ELISA，并就此初步进行方法学验证。结果所建立的ELISA具有良好的特异性、灵敏度、精密度和耐用性，具有替代动物试验方法的良好前景。结论在进一步验证和确认之后，该方法有望正式成为可用于鉴定A型肉毒毒素的试验方法以替代动物试验法。
목적：건립감정A형육독독소적ELISA감별시험방법이체대전통적동물시험법。방법채용현대면역학기술，제비마원성화토원성항A형육독독소다극륭항체，건립료쌍항체협심ELISA，병취차초보진행방법학험증。결과소건립적ELISA구유량호적특이성、령민도、정밀도화내용성，구유체대동물시험방법적량호전경。결론재진일보험증화학인지후，해방법유망정식성위가용우감정A형육독독소적시험방법이체대동물시험법。
Objective To establish a new identification method for botulinum toxin type A ( botA) to replace the traditional animal test.Methods A double antibody sandwich ELISA was established based on the modern immunological technolo-gy, by using prepared two kinds of anti-botA polyclonal antibodies derived from equine and rabbit origin, and a preliminary verification test was performed.Results The established identification ELISA could be a potential alternative method in re-placement of animal test, it is of good specificity, sensitivity, precision and robustness.Conclusion The established method will be expected to be approved for the identification test of botulinum toxin type A as the replacement method for traditional animal test by a further verification and validation.