暨南大学学报(自然科学与医学版)
暨南大學學報(自然科學與醫學版)
기남대학학보(자연과학여의학판)
JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION)
2015年
3期
246-250
,共5页
钟兴%弓健%郭斌%徐浩
鐘興%弓健%郭斌%徐浩
종흥%궁건%곽빈%서호
survivin%RNA 干扰%鼻咽癌%钠/碘同向转运体%碘放射性同位素
survivin%RNA 榦擾%鼻嚥癌%鈉/碘同嚮轉運體%碘放射性同位素
survivin%RNA 간우%비인암%납/전동향전운체%전방사성동위소
survivin%siRNA%sodium /iodide symporter(NIS)%nasopharyngeal carcinoma%i-odine radioisotopes
目的:研究 Survivin 特异性小片段干扰 RNA(siRNA)能否增强人钠碘同向转运体(hNIS)转染的鼻咽癌细胞株(CNE-2-hNIS)对131碘的放射敏感性.方法:设计并合成针对 survivin 基因的特异性 siRNA,利用脂质体法转染CNE-2-hNIS 细胞,通过实时荧光定量聚合酶链式反应(RT-qPCR)和 Western blot 方法检测细胞 survivin 基因沉默效果.CCK-8、克隆形成实验和 AnnexinV FITC /PI 双染流式细胞仪检测131碘孵育后对细胞增殖和凋亡的影响.结果:Survivin siRNA 转染 CNE-2-hNIS 细胞72 h 后,细胞的 survivin 基因 mRNA 和蛋白表达明显降低,Si-survivin 组较 SiR-NA-NC 组细胞增殖率降低,131碘孵育后,Si-survivin 组较 SiRNA-NC 组细胞增殖率降低,凋亡率增高,差别有统计学意义(P <0.05).结论:Survivin 特异性 siRNA 能显著沉默 CNE-2-hNIS 细胞的 survivin 基因的表达,增加 CNE-2-hNIS对131碘的放射敏感性.
目的:研究 Survivin 特異性小片段榦擾 RNA(siRNA)能否增彊人鈉碘同嚮轉運體(hNIS)轉染的鼻嚥癌細胞株(CNE-2-hNIS)對131碘的放射敏感性.方法:設計併閤成針對 survivin 基因的特異性 siRNA,利用脂質體法轉染CNE-2-hNIS 細胞,通過實時熒光定量聚閤酶鏈式反應(RT-qPCR)和 Western blot 方法檢測細胞 survivin 基因沉默效果.CCK-8、剋隆形成實驗和 AnnexinV FITC /PI 雙染流式細胞儀檢測131碘孵育後對細胞增殖和凋亡的影響.結果:Survivin siRNA 轉染 CNE-2-hNIS 細胞72 h 後,細胞的 survivin 基因 mRNA 和蛋白錶達明顯降低,Si-survivin 組較 SiR-NA-NC 組細胞增殖率降低,131碘孵育後,Si-survivin 組較 SiRNA-NC 組細胞增殖率降低,凋亡率增高,差彆有統計學意義(P <0.05).結論:Survivin 特異性 siRNA 能顯著沉默 CNE-2-hNIS 細胞的 survivin 基因的錶達,增加 CNE-2-hNIS對131碘的放射敏感性.
목적:연구 Survivin 특이성소편단간우 RNA(siRNA)능부증강인납전동향전운체(hNIS)전염적비인암세포주(CNE-2-hNIS)대131전적방사민감성.방법:설계병합성침대 survivin 기인적특이성 siRNA,이용지질체법전염CNE-2-hNIS 세포,통과실시형광정량취합매련식반응(RT-qPCR)화 Western blot 방법검측세포 survivin 기인침묵효과.CCK-8、극륭형성실험화 AnnexinV FITC /PI 쌍염류식세포의검측131전부육후대세포증식화조망적영향.결과:Survivin siRNA 전염 CNE-2-hNIS 세포72 h 후,세포적 survivin 기인 mRNA 화단백표체명현강저,Si-survivin 조교 SiR-NA-NC 조세포증식솔강저,131전부육후,Si-survivin 조교 SiRNA-NC 조세포증식솔강저,조망솔증고,차별유통계학의의(P <0.05).결론:Survivin 특이성 siRNA 능현저침묵 CNE-2-hNIS 세포적 survivin 기인적표체,증가 CNE-2-hNIS대131전적방사민감성.
Aim:To explore the effects of siRNA-mediated survivin on the enhancement radiosensitivity of Nasopharyngeal Carcinoma cell transferred Human Sodium /Iodide Symporter Gene (CNE-2-hNIS)to 131 I.Methods:The survivin siRNA was successfully constructed and was transiently transfected into NPC cell line CNE-2-hNIS by liposome-mediated.The expression of survivin was detected by RT-qPCR and western blot.The proliferation and apoptosis of CNE-2-hNIS after treatment with 131 I in vitro were detec-ted by CCK-8 cell proliferation,colony formation assay and Annexin V-FITC /PI double-labeled flow cy-tometry.Results:Significant down-regulation of the survivin protein expression was found after transfec-tion of the survivin-siRNA in CNE-2-hNIS cells.With 131 I treatment,the rate of proliferation in survivin-siRNA group was gradually lesser,whereas the rate of cell apoptosis was gradually increased than those of SiRNA-NC group.Conclusion:The survivin special siRNA silenced survivin gene of CNE-2-hNIS and en-hanced the radiosensitivity of CNE-2-hNIS to 131 I.