暨南大学学报(自然科学与医学版)
暨南大學學報(自然科學與醫學版)
기남대학학보(자연과학여의학판)
JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION)
2015年
3期
233-238
,共6页
汤颖聪%杨睿%李红%李启艳
湯穎聰%楊睿%李紅%李啟豔
탕영총%양예%리홍%리계염
小白菊内酯%牙周炎%脂多糖%细胞因子%抗炎
小白菊內酯%牙週炎%脂多糖%細胞因子%抗炎
소백국내지%아주염%지다당%세포인자%항염
parthenolide%periodontitis%lipopolysaccharide%cytokine%anti-inflammation
目的:研究小白菊内酯对细菌脂多糖诱导炎症反应的抑制作用.方法:选用小鼠巨噬细胞 RAW264.7,采用实时定量多聚酶链式反应及酶联免疫吸附法,检测不同浓度小白菊内酯(0.1、1、10、50μmol/L)和细菌脂多糖(10μmol/L)作用后,细胞因子的 mRNA(IL-6、TNF-α、Ptgs2、IL-1β、IL-10、MMP13与 CXCL1)及蛋白(IL-6、TNF-α、IL-1β与 IL-10)表达水平的改变.实验同时设立4个对照组,包括 DMSO 组、脂多糖组、DMSO +脂多糖组及阳性药Bay 11-7082+脂多糖组.结果:浓度分别为10和50μmol/L 小白菊内酯稳定有效地抑制脂多糖诱导小鼠巨噬细胞产生细胞因子.在 mRNA 水平上,IL-6、TNF-α、Ptgs2、IL-1β、IL-10与 MMP13的 mRNA 表达水平明显下降,差异有显著性,CXCL1 mRNA 表达水平改变无统计学差异;在蛋白水平上,IL-6、TNF-α、IL-1β与 IL-10的表达亦显著性下降,有统计学差异.结论:小白菊内酯有效抑制了细菌脂多糖诱导的小鼠巨噬细胞中炎症细胞因子的表达.
目的:研究小白菊內酯對細菌脂多糖誘導炎癥反應的抑製作用.方法:選用小鼠巨噬細胞 RAW264.7,採用實時定量多聚酶鏈式反應及酶聯免疫吸附法,檢測不同濃度小白菊內酯(0.1、1、10、50μmol/L)和細菌脂多糖(10μmol/L)作用後,細胞因子的 mRNA(IL-6、TNF-α、Ptgs2、IL-1β、IL-10、MMP13與 CXCL1)及蛋白(IL-6、TNF-α、IL-1β與 IL-10)錶達水平的改變.實驗同時設立4箇對照組,包括 DMSO 組、脂多糖組、DMSO +脂多糖組及暘性藥Bay 11-7082+脂多糖組.結果:濃度分彆為10和50μmol/L 小白菊內酯穩定有效地抑製脂多糖誘導小鼠巨噬細胞產生細胞因子.在 mRNA 水平上,IL-6、TNF-α、Ptgs2、IL-1β、IL-10與 MMP13的 mRNA 錶達水平明顯下降,差異有顯著性,CXCL1 mRNA 錶達水平改變無統計學差異;在蛋白水平上,IL-6、TNF-α、IL-1β與 IL-10的錶達亦顯著性下降,有統計學差異.結論:小白菊內酯有效抑製瞭細菌脂多糖誘導的小鼠巨噬細胞中炎癥細胞因子的錶達.
목적:연구소백국내지대세균지다당유도염증반응적억제작용.방법:선용소서거서세포 RAW264.7,채용실시정량다취매련식반응급매련면역흡부법,검측불동농도소백국내지(0.1、1、10、50μmol/L)화세균지다당(10μmol/L)작용후,세포인자적 mRNA(IL-6、TNF-α、Ptgs2、IL-1β、IL-10、MMP13여 CXCL1)급단백(IL-6、TNF-α、IL-1β여 IL-10)표체수평적개변.실험동시설립4개대조조,포괄 DMSO 조、지다당조、DMSO +지다당조급양성약Bay 11-7082+지다당조.결과:농도분별위10화50μmol/L 소백국내지은정유효지억제지다당유도소서거서세포산생세포인자.재 mRNA 수평상,IL-6、TNF-α、Ptgs2、IL-1β、IL-10여 MMP13적 mRNA 표체수평명현하강,차이유현저성,CXCL1 mRNA 표체수평개변무통계학차이;재단백수평상,IL-6、TNF-α、IL-1β여 IL-10적표체역현저성하강,유통계학차이.결론:소백국내지유효억제료세균지다당유도적소서거서세포중염증세포인자적표체.
Aim:To study the inhibitory effects of parthenolide on LPS induced cytokine expression in RAW264.7 cells.Methods:Quantitative reverse transcription real-time polymerase chain reaction and enzyme linked immunosorbent assay were used to determine some cytokines’expression at mRNA levels (IL-6,TNF-α,Ptgs2,IL-1β,IL-10,MMP13 and CXCL1)and at protein levels (IL-6,TNF-α,IL-1βand IL-10)after treatment of parthenolide (0.1,1,10,50 μmol /L)and Lipopolysaccharide (LPS,10 <br> μmol /L)in RAW264.7 cells.Four control groups were included in the study.They were DMSO group, LPS group,DMSO +LPS group,and Bay 11-7082 +LPS group.Results:The mRNA expression levels of IL-6,TNF-α,Ptgs2,IL-1β,IL-10 and MMP13 were significantly decreased by 10 or 50 μmol /L parthe-nolide after RAW264.7 cells were treated with 10 μmol /L LPS for 8 hours.However,no significant change of CXCL1 in mRNA expression level was observed.The protein levels of IL-6,TNF-α,IL-1βand IL-10 were also found to be inhibited by 10 or 50 μmol /L parthenolide after RAW264.7 cells were trea-ted with 10 μmol /L LPS for 24 hours.Conclusion:Cytokines’expression induced by LPS in RAW264.7 cells was arrested by parthenolide.