CAJ | 학술논문

目的：比较研究和评价富硒螺旋藻（SeSP）生物转化产生的主要富硒活性形式在体外清除自由基的活性．方法：利用富硒培养技术制备 SeSP，从 SeSP 中分级提取纯化硒总蛋白（SeSP-TP）、硒藻胆蛋白复合体（SeSP-PB）、硒藻蓝蛋白（SeSP-PC）、硒多糖（SeSP-PS）和纳米红硒（Nano-Se）；电感耦合等离子质谱仪（ICP-MS）测定硒含量；超氧阴离子（·O2）和羟基自由基（·OH）化学发光体系检测自由基清除率，Reed-Muench 算法计算半数有效浓度（EC50）．结果：SeSP-TP、SeSP-PB 和 SeSP-PC 与不含硒相同形态对自由基的清除作用均显著提高（P ＜0.05），对·O2和·OH 清除作用的 EC50明显降低（P ＜0.05），SeSP 中 SeSP-PB 在体外对自由基的清除活性最高，其中SeSP-PC 可能起主要作用；SeSP-PS 硒的质量分数低，其中硒对自由基清除的增强效应尚不明确；微藻转化的 Nano-Se 也具有较强的自由基清除活性．结论：SeSP 生物转化富硒活性形式对自由基清除的增强效应与活性硒元素的掺入有关，藻体转化生成的富硒蛋白质产生了协同增效作用明显，生物转化纳米硒作为一种新型活性硒形态值得深入研究．
목적：비교연구화평개부서라선조（SeSP）생물전화산생적주요부서활성형식재체외청제자유기적활성．방법：이용부서배양기술제비 SeSP，종 SeSP 중분급제취순화서총단백（SeSP-TP）、서조담단백복합체（SeSP-PB）、서조람단백（SeSP-PC）、서다당（SeSP-PS）화납미홍서（Nano-Se）；전감우합등리자질보의（ICP-MS）측정서함량；초양음리자（·O2）화간기자유기（·OH）화학발광체계검측자유기청제솔，Reed-Muench 산법계산반수유효농도（EC50）．결과：SeSP-TP、SeSP-PB 화 SeSP-PC 여불함서상동형태대자유기적청제작용균현저제고（P ＜0.05），대·O2화·OH 청제작용적 EC50명현강저（P ＜0.05），SeSP 중 SeSP-PB 재체외대자유기적청제활성최고，기중SeSP-PC 가능기주요작용；SeSP-PS 서적질량분수저，기중서대자유기청제적증강효응상불명학；미조전화적 Nano-Se 야구유교강적자유기청제활성．결론：SeSP 생물전화부서활성형식대자유기청제적증강효응여활성서원소적참입유관，조체전화생성적부서단백질산생료협동증효작용명현，생물전화납미서작위일충신형활성서형태치득심입연구．
Aim:To investigate the scavenging effects of selenium (Se)containing specimen from Se enriched Spirulina platensis (SeSP)on free radicals in vitro.Methods:SeSP was cultivated and pre-pared by the optimized procedure with Se-enriched medium.Specimen of Se containing total protein (SeSP-TP),phycobiliprotein complexes (SeSP-PB),phycocyanin (SeSP-PC),polysaccharides (SeSP-PS)and nano red elemental Se (Nano-Se)were isolated and purified from SeSP.Se contents were meas-ured by the inductively coupled plasma mass spectrometry (ICP-MS)instrument after microwave diges-tion.The scavenging activity of different Se containing specimen on superoxide anions (·O2 )and hy-droxyl radicals (·OH)were monitored by chemiluminescence method in vitro,and the median effect concentration (EC50)was calculated by Reed - Muench algorithm.Results:The scavenging activities of SeSP-TP,SeSP-PB,and SeSP-PC on ·O2 and ·OH were all elevated significantly (P <0.05 ), which was clearly in consistent with the decreased EC50s to ·O2 and ·OH scavenging in comparison with those of specimen from SP.Among the Se containing specimen in SeSP,SeSP-PB was confirmed to be the strongest scavenger and SeSP-PC served as a dominant player in scavenging radicals.As a novel active elemental Se species,Nano-Se bio-transformed from SeSP was detected high potent to radicals re-moving.While,the SeSP-PS was found no obviously enhancement on the radicals scavenging activity due to the low Se content binding to PS molecular.Conclusion:Se inserting into the specimen,especially the proteins by bio-transformation,result in a synergistic effect of the antioxidants to clean out radicals.In prospective,activities and medical values of the novel bio-transformed Nano-Se should be explored in fur-ther.