牙体牙髓牙周病学杂志
牙體牙髓牙週病學雜誌
아체아수아주병학잡지
CHINESE JOURNAL OF CONSERVATIVE DENTISTRY
2015年
6期
354-358,341
,共6页
牙周缓释制剂%睾丸%超微病理%组织酶%基因表达
牙週緩釋製劑%睪汍%超微病理%組織酶%基因錶達
아주완석제제%고환%초미병리%조직매%기인표체
periodontal sustained release preparation%testis%ultrastructural pathology%tissue enzymes%gene expression
目的：：观察复方奥硝唑甲磺酸培氟沙星( COPM)牙周缓释制剂对大鼠睾丸毒性的作用机制。方法：将40只SD雄性大鼠随机分为阴性对照组和低、中、高剂量组( n=10),每日分别按1、4、8 g/kg-1的剂量灌服COPM牙周缓释制剂,阴性对照组每日按体质量灌服相应体积的双蒸水,连续42 d。所有大鼠处死后,分别计算附睾系数,检查精子计数、活动率和畸形率；透射电镜观察生精细胞的超微结构；考马斯亮蓝法测定睾丸组织中乳酸脱氢酶( LDH)、碱性磷酸酶( ALP)、酸性磷酸酶( ACP )、γ-谷氨酰转肽酶(γ-GT)的活力；RT-PCR检测睾丸组织中雄激素结合蛋白( ABP)、抑制素-α( inhibin-α)基因的mRNA表达水平。结果：高剂量组睾丸、附睾系数减小,精子活力降低、计数减少、畸形率增加(P<0.05),生精细胞超微结构出现不同程度损害；高剂量组睾丸组织中的LDH、LAL、ACP和高、中剂量组的γ-GT活力降低；高剂量组睾丸组织中的ABP和inhibin-α mRNA表达水平下降(P<0.05)。结论：高剂量COPM牙周缓释制剂对大鼠睾丸有一定的毒性；睾丸组织中LDH、ALP、ACP、γ-GT的活力下降,ABP和inhibin-αmRNA的表达减少可能是其毒性作用机制之一。
目的：：觀察複方奧硝唑甲磺痠培氟沙星( COPM)牙週緩釋製劑對大鼠睪汍毒性的作用機製。方法：將40隻SD雄性大鼠隨機分為陰性對照組和低、中、高劑量組( n=10),每日分彆按1、4、8 g/kg-1的劑量灌服COPM牙週緩釋製劑,陰性對照組每日按體質量灌服相應體積的雙蒸水,連續42 d。所有大鼠處死後,分彆計算附睪繫數,檢查精子計數、活動率和畸形率；透射電鏡觀察生精細胞的超微結構；攷馬斯亮藍法測定睪汍組織中乳痠脫氫酶( LDH)、堿性燐痠酶( ALP)、痠性燐痠酶( ACP )、γ-穀氨酰轉肽酶(γ-GT)的活力；RT-PCR檢測睪汍組織中雄激素結閤蛋白( ABP)、抑製素-α( inhibin-α)基因的mRNA錶達水平。結果：高劑量組睪汍、附睪繫數減小,精子活力降低、計數減少、畸形率增加(P<0.05),生精細胞超微結構齣現不同程度損害；高劑量組睪汍組織中的LDH、LAL、ACP和高、中劑量組的γ-GT活力降低；高劑量組睪汍組織中的ABP和inhibin-α mRNA錶達水平下降(P<0.05)。結論：高劑量COPM牙週緩釋製劑對大鼠睪汍有一定的毒性；睪汍組織中LDH、ALP、ACP、γ-GT的活力下降,ABP和inhibin-αmRNA的錶達減少可能是其毒性作用機製之一。
목적：：관찰복방오초서갑광산배불사성( COPM)아주완석제제대대서고환독성적작용궤제。방법：장40지SD웅성대서수궤분위음성대조조화저、중、고제량조( n=10),매일분별안1、4、8 g/kg-1적제량관복COPM아주완석제제,음성대조조매일안체질량관복상응체적적쌍증수,련속42 d。소유대서처사후,분별계산부고계수,검사정자계수、활동솔화기형솔；투사전경관찰생정세포적초미결구；고마사량람법측정고환조직중유산탈경매( LDH)、감성린산매( ALP)、산성린산매( ACP )、γ-곡안선전태매(γ-GT)적활력；RT-PCR검측고환조직중웅격소결합단백( ABP)、억제소-α( inhibin-α)기인적mRNA표체수평。결과：고제량조고환、부고계수감소,정자활력강저、계수감소、기형솔증가(P<0.05),생정세포초미결구출현불동정도손해；고제량조고환조직중적LDH、LAL、ACP화고、중제량조적γ-GT활력강저；고제량조고환조직중적ABP화inhibin-α mRNA표체수평하강(P<0.05)。결론：고제량COPM아주완석제제대대서고환유일정적독성；고환조직중LDH、ALP、ACP、γ-GT적활력하강,ABP화inhibin-αmRNA적표체감소가능시기독성작용궤제지일。
AIM: To observe the testicular influence of compound ornidazole and pefloxacin mesylate ( COPM) of periodontal sustained release preparation on male rats and related mechanism. METHODS:40 male SD rats were divided into negative control, low, medium and high dose groups by random digit table(n=10) and were fed daily with COPM of the preparation at 1,4 and 8 g. kg-1 respectively, those in the negative group were fed daily by distilled water, continuously for 42 d. The coefficient of testis and epididymis, motility, count and deformity of sperm were exam-ined, ultrastructural examination on sperm atogenic cells were respectively observed;LDH, ALP, ACP,γ-GT in testicu-lar tissue were determined respectively, the mRNA expression of ABP and inhibin - α in testis was examtned. RESULTS:In high dose group the coefficient of testis and epididymis, motility, count and deformity of sperm were all decreased (P<0. 05);ultrastructure was changed; LDH, ALP, ACP, γ-GT, ABP and inhibin-α were all decreased (P<0. 05). CONCLUSION:COPM of periodontal sustained release preparations at high dose has toxicity on testis of male SD rats, the reduction of LDH, ALP, ACP,γ-GT, ABP and inhibin-αin testis may be the cause of mechanisms.
