特产研究
特產研究
특산연구
SPECIAL WILD ECONOMIC ANIMAL AND PLANT RESEARCH
2015年
2期
65-69
,共5页
五味子%组织培养%外植体%培养基%展望
五味子%組織培養%外植體%培養基%展望
오미자%조직배양%외식체%배양기%전망
Schisandra chinensis (Turcz .) Baill%tissue culture%explant%culture medium%prospect
综述了近年来五味子组织培养研究进展,对不同培养体系进行了总结与分析。五味子组织培养主要以MS、B5及N6等为基本培养基,附加激素以BA (6-苄氨基腺嘌呤)、NAA (α-萘乙酸)等为主,但激素浓度与比例差异较大;来自不同地区的不同种质外植体,其芽苗与愈伤诱导率差异较大,茎段(春芽与冬芽)是芽苗、愈伤诱导及快速繁育较为适宜的外植体;应加强新品种或当地优系的组织培养快速繁育体系研究,开展五味子优良品种、品系组织培养长期保存研究,保护优异资源;以胚状体方式快速繁育等研究是新品种快速发酵工厂化育苗研究的新动向。
綜述瞭近年來五味子組織培養研究進展,對不同培養體繫進行瞭總結與分析。五味子組織培養主要以MS、B5及N6等為基本培養基,附加激素以BA (6-芐氨基腺嘌呤)、NAA (α-萘乙痠)等為主,但激素濃度與比例差異較大;來自不同地區的不同種質外植體,其芽苗與愈傷誘導率差異較大,莖段(春芽與鼕芽)是芽苗、愈傷誘導及快速繁育較為適宜的外植體;應加彊新品種或噹地優繫的組織培養快速繁育體繫研究,開展五味子優良品種、品繫組織培養長期保存研究,保護優異資源;以胚狀體方式快速繁育等研究是新品種快速髮酵工廠化育苗研究的新動嚮。
종술료근년래오미자조직배양연구진전,대불동배양체계진행료총결여분석。오미자조직배양주요이MS、B5급N6등위기본배양기,부가격소이BA (6-변안기선표령)、NAA (α-내을산)등위주,단격소농도여비례차이교대;래자불동지구적불동충질외식체,기아묘여유상유도솔차이교대,경단(춘아여동아)시아묘、유상유도급쾌속번육교위괄의적외식체;응가강신품충혹당지우계적조직배양쾌속번육체계연구,개전오미자우량품충、품계조직배양장기보존연구,보호우이자원;이배상체방식쾌속번육등연구시신품충쾌속발효공엄화육묘연구적신동향。
The status of tissue culture were summarized in Schisandra chinensis(Turcz .) Baill through analysis of the culture system .MS ,B5 or N6 was used as main basic medium supplemented with variable contents of phytohormones of BA and/or NAA .the inducing rate of bud cluster and callus were both different among explants of diverse germ plasms in vary regions and the stem was the suitable explant for callus inducing and rapid generation .Tissue culture was appropriate method for rapid generation and preservation of new varieties and strains .Embryoid culture would be the tendency in the industrialized seedling production of new variety .