CAJ | 학술논문

实蝇类害虫严重危害多种水果和蔬菜，是世界果蔬产业最重要的害虫类群之一，严重影响了发生地的果蔬生产和出口贸易活动。昆虫不育技术( SIT)是一种物种特异和环境友好型防治措施，在多种实蝇类害虫的防治、阻截和根除中起到了不可替代的重要作用。通过分子生物学技术对昆虫的基因组进行遗传修饰，可对SIT进行改进，提高其防控效果并扩大应用的物种范围，近年来相关方面的研究已取得重要进展，成为害虫遗传控制的研究热点。本文阐述了通过受四环素调控的tet-off基因表达系统来实现昆虫“不育”的基本原理和在果蝇及其他几种主要实蝇类害虫中建立的不同类型的遗传控制体系，以及类似体系在其他农业昆虫中的应用情况。简要介绍了在橘小实蝇遗传控制技术体系构建方面的工作进展，并对该技术的在害虫综合治理( IPM)尤其是实蝇类害虫防治中的应用前景进行了讨论和展望。
실승류해충엄중위해다충수과화소채，시세계과소산업최중요적해충류군지일，엄중영향료발생지적과소생산화출구무역활동。곤충불육기술( SIT)시일충물충특이화배경우호형방치조시，재다충실승류해충적방치、조절화근제중기도료불가체대적중요작용。통과분자생물학기술대곤충적기인조진행유전수식，가대SIT진행개진，제고기방공효과병확대응용적물충범위，근년래상관방면적연구이취득중요진전，성위해충유전공제적연구열점。본문천술료통과수사배소조공적tet-off기인표체계통래실현곤충“불육”적기본원리화재과승급기타궤충주요실승류해충중건립적불동류형적유전공제체계，이급유사체계재기타농업곤충중적응용정황。간요개소료재귤소실승유전공제기술체계구건방면적공작진전，병대해기술적재해충종합치리( IPM)우기시실승류해충방치중적응용전경진행료토론화전망。
Many tephritid fruit flies are economically important pest species that can destroy fruits and vegetables, bringing can be a barrier to international trades. The Sterile Insect Technique ( SIT) , a species-specific and environment-friendly pest control method, has been proven to be highly effective against tephritid pests. Genetic engineering has a potential to bring great improvements to SIT and facilitate its applicability to more insect pests. Much progress has been made in the area, which also became a frontier of genetic innovation. Here we describe the principles of lethality systems based on the tet-off inducible gene expression system, same case studies from Drosophila and tephritid fruit flies for the improvement of SIT, and the application of similar systems to other agricultur-al insects. The progress to develop a genetic control system on Bactrocera dorsalis is also briefly reported here. The genetic control strategies based on genetic engineering and SIT is believed to play a great role in the Integrated Pest Management ( IPM) of tephritid fruit flies and other agricultural insect pests in the future.