CAJ | 학술논문

本实验建立了茵山莲软胶囊中栀子苷含量的测定方法。采用反相高效液相色谱法，选择迪马C18色谱柱（4.6mm ×250mm ，5μm ），以乙腈－水（V乙腈∶V水＝13∶87）为流动相，检测波长238nm ，流速1.0mL／min ，柱温25℃，进样量10μL。栀子苷在0.00804mg／mL～0.0804mg／mL范围内质量浓度与峰面积呈良好的线性关系，R2＝0.9998，平均回收率97.667％，RSD 0.567％（ n＝6）。该试验的测定方法简单准确，且分离效果好，灵敏度较高，可作为茵山莲软胶囊的质量控制方法。
본실험건립료인산련연효낭중치자감함량적측정방법。채용반상고효액상색보법，선택적마C18색보주（4.6mm ×250mm ，5μm ），이을정－수（V을정∶V수＝13∶87）위류동상，검측파장238nm ，류속1.0mL／min ，주온25℃，진양량10μL。치자감재0.00804mg／mL～0.0804mg／mL범위내질량농도여봉면적정량호적선성관계，R2＝0.9998，평균회수솔97.667％，RSD 0.567％（ n＝6）。해시험적측정방법간단준학，차분리효과호，령민도교고，가작위인산련연효낭적질량공제방법。
The present study developed a method to determine the levels of geniposide in Yinshanlian soft capsules with RP-HPLC .Geniposide was performed on a Diamonsil C18 column (4.6mm × 250mm ,5μm ) with the mobile phase of acetonit water (V∶V=13∶87 ) .The detection wavelength was set at 238 nm and the column temperature was 25℃ .There had a good linear relationship between geniposide contents and peak area when its concentration localized in the range from 0.008 04mg/mL to 0.0804mg/mL .The recovery rate of geniposide was 97.667% ,RSD were 0.567% .The method we used has the characteristics of simplicity ,accuracy ,as well as high efficency and sensitivity .Thus it can be used to test the quality of Yinshanlian soft capsules .