CAJ | 학술논문

根据 EST 序列设计引物，应用 RACE 和 RT －PCR 技术从橡胶树的胶乳（乳管细胞的胞质成分）中分离到1条长2102 bp 的 cDNA，该序列包含1个1803 bp 的开放读码框（ORF），81 bp 的5’ UTR 和218 bp 的3’UTR；ORF 预测编码600个氨基酸，其理论分子量为67．88 kDa，等电点为8．56，质体定位；蛋白含有多酚氧化酶特有的 Tyrosinase、PPO1＿DWL 和 PPO1＿KFDV 结构域；蛋白与木薯、蓖麻、麻疯树和胡杨中同源蛋白的相似性分别为85．2％、82．0％、79．8％和78．2％，将基因命名为 HbPPO1。结果表明，在所分析的胶乳、树皮和叶片组织中，基因在胶乳中的表达丰度最高，且其表达水平受乙烯利显著抑制。
근거 EST 서렬설계인물，응용 RACE 화 RT －PCR 기술종상효수적효유（유관세포적포질성분）중분리도1조장2102 bp 적 cDNA，해서렬포함1개1803 bp 적개방독마광（ORF），81 bp 적5’ UTR 화218 bp 적3’UTR；ORF 예측편마600개안기산，기이론분자량위67．88 kDa，등전점위8．56，질체정위；단백함유다분양화매특유적 Tyrosinase、PPO1＿DWL 화 PPO1＿KFDV 결구역；단백여목서、비마、마풍수화호양중동원단백적상사성분별위85．2％、82．0％、79．8％화78．2％，장기인명명위 HbPPO1。결과표명，재소분석적효유、수피화협편조직중，기인재효유중적표체봉도최고，차기표체수평수을희리현저억제。
Based on the EST sequence,a 2 102 bp cDNA was isolated from the rubber tree (Hevea brasilien-sis)latex (representing the laticicfer cytoplasm)with specific primers via RACE and RT -PCR.The sequence designated as HbPPO1 contains a 1 803 bp long open reading frame (ORF),81 bp 5′UTR and 218 bp 3′UTR. Sequence analysis indicated that HbPPO1 putatively encodes 600 amino acids with a theoretical molecular weight (Mw)of 67.88 kDa and isolectric point (pI)of 8.56,which was predicted to be localized to the plastid.The pro-tein contains the conserved Tyrosinase,PPO1 _DWL and PPO1 _KFDV domains specific to polyphenol oxidases, and shares the similarity of 85.2%,82.0%,79.8% and 78.2% with its homologs in Manihot esculenta,Ricinus communis,Jatropha curcas and Populus euphratica.Expression analysis showed that,among the examined tissues such as laticifer,bark and leaf,HbPPO1 expressed relatively more in the laticifer and the expression level was sig-nificantly inhibited by ethylene.