CAJ | 학술논문

目的：探讨系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMCs）中干扰素（IFN）诱导基因MNDA mRNA 的表达及其与 SLE 疾病活动度的相关性。方法应用半定量聚合酶链反应（RT-PCR）方法检测30例 SLE 患者与25例健康对照者的 MNDA mRNA 表达水平，并且分析其与尿蛋白、补体 C3、C4、抗 dsDNA抗体及 SLE 疾病活动指数（SLEDAI）积分的相关性。结果SLE 组与对照组相比，MNDA mRNA 表达显著增高（t ＝6．99，P ＜0．01）；SLE 组尿蛋白阳性患者的 MNDA mRNA 表达水平较尿蛋白阴性患者显著增高（t ＝3.08，P ＜0．01），尿蛋白阳性组及阴性组 AIM2 mRNA 表达水平均显著高于对照组（t ＝9．32、4．87，均 P ＜0.01）；SLE 组 MNDA mRNA 表达水平与抗 dsDNA 抗体、SLEDAI 积分呈显著正相关（r ＝0．534、0．508，均 P ＜0．05），与补体 C3、C4不相关（r ＝－0．472、－0．349，均 P ＞0．05）。结论SLE 患者 MNDA mRNA 的表达水平显著增高，并与肾脏损伤、疾病活动度相关，检测该指标对 SLE 患者的诊断和病情评估有较大价值。
목적：탐토계통성홍반랑창（SLE）환자외주혈단개핵세포（PBMCs）중간우소（IFN）유도기인MNDA mRNA 적표체급기여 SLE 질병활동도적상관성。방법응용반정량취합매련반응（RT-PCR）방법검측30례 SLE 환자여25례건강대조자적 MNDA mRNA 표체수평，병차분석기여뇨단백、보체 C3、C4、항 dsDNA항체급 SLE 질병활동지수（SLEDAI）적분적상관성。결과SLE 조여대조조상비，MNDA mRNA 표체현저증고（t ＝6．99，P ＜0．01）；SLE 조뇨단백양성환자적 MNDA mRNA 표체수평교뇨단백음성환자현저증고（t ＝3.08，P ＜0．01），뇨단백양성조급음성조 AIM2 mRNA 표체수평균현저고우대조조（t ＝9．32、4．87，균 P ＜0.01）；SLE 조 MNDA mRNA 표체수평여항 dsDNA 항체、SLEDAI 적분정현저정상관（r ＝0．534、0．508，균 P ＜0．05），여보체 C3、C4불상관（r ＝－0．472、－0．349，균 P ＞0．05）。결론SLE 환자 MNDA mRNA 적표체수평현저증고，병여신장손상、질병활동도상관，검측해지표대 SLE 환자적진단화병정평고유교대개치。
Objective To investigate the expression levels of interferon -inducible genes (MNDA)in the peripheral blood mononuclear cells (PBMCs)of patients with systemic lupus erythematosus (SLE),and the relations between the gene expression levels and disease activity were explored.Methods Reverse transcription polymerase chain reaction(RT -PCR)method was used to detect the expression levels of MNDA in 30 patients with SLE and 25 controls.The associations between the expression levels of MNDA and urine protein,complement C3,C4,anti -double stranded DNA antibody and SLEDAI scores in patients with SLE were analyzed.Results (1)The expression level of MNDA mRNA in the SLE patients was significantly higher than that of the normal controls(t =6.99,P <0.01).(2)The expression level of MNDA in the urinary protein positive patients was significantly higher than that of the urinary protein negative patients (t =3.08,P <0.01),they were all significantly higher than the normal controls (t =9.32,4.87,all P <0.01).(3)The expression levels of MNDA mRNA were positively correlated with anti -double stranded DNA antibody and SLEDAI scores(r =0.534,0.508,all P <0.05),and not correlated with comple-ment C3 and C4(r =-0.472,-0.349,all P >0.05 ).Conclusion The expression levels of MNDA mRNA in patients with SLE are significantly higher than those of the normal controls.It is linked to kidney damage and disease activity,and helpful in evaluating SLE disease activity and severity.