CAJ | 학술논문

为了研究明绿豆中苯丙氨酸解氨酶（PAL）的动力学特性，采用35％和65％饱和度的硫酸铵分级沉淀、DE -52阴离子交换层析方法对明绿豆中的 PAL 进行了分离纯化，并对其基本性质做了初步研究。结果表明，PAL 的纯化倍数为6.9829，蛋白质得率为4.65％，酶得率为32.45％。PAL 在硼砂-硼酸缓冲溶液中适宜的 pH 范围为7.6～9.0，最适 pH 有2个：8.0和8.6。PAL 适宜的温度范围为35～45℃，最适反应温度为40℃。得到 PAL 的2个 Km值，分别是：Km1为6.94×10-5 mol ／L，Km2为1.23×10-4 mol ／L。本研究结果可为进一步研究和开发明绿豆的 PAL 提供参考。
위료연구명록두중분병안산해안매（PAL）적동역학특성，채용35％화65％포화도적류산안분급침정、DE -52음리자교환층석방법대명록두중적 PAL 진행료분리순화，병대기기본성질주료초보연구。결과표명，PAL 적순화배수위6.9829，단백질득솔위4.65％，매득솔위32.45％。PAL 재붕사-붕산완충용액중괄의적 pH 범위위7.6～9.0，최괄 pH 유2개：8.0화8.6。PAL 괄의적온도범위위35～45℃，최괄반응온도위40℃。득도 PAL 적2개 Km치，분별시：Km1위6.94×10-5 mol ／L，Km2위1.23×10-4 mol ／L。본연구결과가위진일보연구화개발명록두적 PAL 제공삼고。
The study in this paper aimed to research the dynamics characteristics of phenylalanine ammonia -lyase (PAL)from Ming mung bean.The isolation,purification and basic properties of L -phenylalanine ammonia -lyase in Ming mung bean were studied by 35% and 65% saturation of ammonium sulfate grade deposition,DE -52 anion exchange chromatography.The results showed that the purification multiple was 6.982 9 and protein yield and PAL yield were 4.65% and 32.45% respectively.The optimal pH range of PAL in borax -boric acid buffer was 7.6 ~9.0 and there were two optimal pH as 8.0 and 8.6.The optimal temperature range of PAL was 35 ~45 ℃. The optimal temperature was 40 ℃.There were two Km values including 6.94 ×10 -5 mol /L and 1.23 ×10 -4 mol /L. The results provided the reference for further studying and developing PAL of Ming mung bean.