CAJ | 학술논문

采用响应面法优化了超临界 CO2流体萃取（SCE）香榧假种皮提取物的工艺，并采用气相色谱质谱联用仪（GC -MS）对其提取物成分进行了分析。在单因素试验的基础上，选取动态萃取时间、萃取温度、萃取压力作为变量，以香榧假种皮提取物得率为指标，采用 Box -Behnken 试验设计方法进行í因素í水平试验，根据回归方程的预测模型对结果进行响应面分析，确定超临界 CO2流体萃取香榧假种皮提取物的优化工艺为：粉碎度40目，静态萃取时间20 min，动态萃取时间2.5 h、萃取温度45℃、萃取压力34 MPa，在此条件下提取物平均提取率为（22.12±0.09）％。经过 GC -MS 检测分析，香榧假种皮提取物的主要成分为二萜类物质，其次为羧酸、酯类、倍半萜类和单萜类物质。
채용향응면법우화료초림계 CO2류체췌취（SCE）향비가충피제취물적공예，병채용기상색보질보련용의（GC -MS）대기제취물성분진행료분석。재단인소시험적기출상，선취동태췌취시간、췌취온도、췌취압력작위변량，이향비가충피제취물득솔위지표，채용 Box -Behnken 시험설계방법진행í인소í수평시험，근거회귀방정적예측모형대결과진행향응면분석，학정초림계 CO2류체췌취향비가충피제취물적우화공예위：분쇄도40목，정태췌취시간20 min，동태췌취시간2.5 h、췌취온도45℃、췌취압력34 MPa，재차조건하제취물평균제취솔위（22.12±0.09）％。경과 GC -MS 검측분석，향비가충피제취물적주요성분위이첩류물질，기차위최산、지류、배반첩류화단첩류물질。
A response surface method (RSM)was used to optimize supercritical CO2 extraction (SCE)of the extracts from Chinese Torreya aril and the main components of aril extracts was analyzed by gas chromatograph -mass spectrometer (GC -MS).Based on the single factor experiments,the dynamic extraction time,extraction tempera-ture and extraction pressure were selected as variables.The extraction yield of Chinese Torreya aril was regarded as index and the three -factor and three -level experiment was conducted by Box -Behnke experiment design method. The optimization process of extracts of Chinese Torreya aril by supercritical CO2 extraction was particle size of 40 mush,extraction time of 20 min,dynamic time of 2.5 h,temperature of 45 ℃ and pressure of 34 MPa.The optimal yield (22.12 ±0.09%)was obtained at the operating conditions.The results of GC -MS analysis showed that the main complements of aril extracts were diterpenoids,fatty acid,followed by sesquiterpenoids and monoterpenoids.