CAJ | 학술논문

采用气相-质谱联用仪（GC -MS）分析了火龙果籽油的脂肪酸组成；利用噻唑蓝（MTT）法，检测了火龙果籽油抑制 HepG -2、HT -29人体癌细胞的增殖，并绘制了火龙果籽油对这些癌细胞生长曲线的影响。结果表明：火龙果籽油中主要含有亚油酸（41.28％）、油酸（26.17％）、棕榈酸（20.76％）等组分，不饱和脂肪酸高达72.10％；MTT 法显示火龙果籽油呈剂量（1、3、5 mg ／mL）和时间（24、48、72、96 h）依赖方式抑制 HepG -2、HT -29等细胞增殖，作用48 h 细胞抑制率分别可达到24.76％、38.92％、48.18％和35.47％、46.11％、53.78％，火龙果籽油对这些癌细胞的生长具有显著性、持续性的抑制作用。
채용기상-질보련용의（GC -MS）분석료화룡과자유적지방산조성；이용새서람（MTT）법，검측료화룡과자유억제 HepG -2、HT -29인체암세포적증식，병회제료화룡과자유대저사암세포생장곡선적영향。결과표명：화룡과자유중주요함유아유산（41.28％）、유산（26.17％）、종려산（20.76％）등조분，불포화지방산고체72.10％；MTT 법현시화룡과자유정제량（1、3、5 mg ／mL）화시간（24、48、72、96 h）의뢰방식억제 HepG -2、HT -29등세포증식，작용48 h 세포억제솔분별가체도24.76％、38.92％、48.18％화35.47％、46.11％、53.78％，화룡과자유대저사암세포적생장구유현저성、지속성적억제작용。
The ingredients and contents of fatty acids in Hylocereus Undatus Britt seed oil (HUSO)were deter-mined with GC -MS.The inhibitory effects of HUSO on the proliferation activities,growth curve of hunman cancer cell line HepG -2 and HT -29 were examined.In addition,the curve about inhibitory effects of HUSO on cancer cell growth was drawed.The results showed that the main fatty acids in HUSO were linolic acid (41.28%),oleie acid (26.17%),and palmitic acid (20.76%).The unsaturated fatty acids were 72.10% of total fatty acids,and the inhibitions of UHSO on the proliferation of HepG -2 and HT -29 were in dose -(1,3 and 5 mg/mL)and time-(24,48,72 and 96 h)dependent manners,and the inhibition rate of HepG -2 and HT -29 cells reached 24.76%,38.92% and 48.18%,35.47%,46.11% and 53.78% after incubation of 48 h respectively.The Hylo-cereus Undatus Britt seed oil had an significant and continued inhibiting effect on the growth of cancer cells.