CAJ | 학술논문

为建立检测病毒性出血性败血症病毒（VHSV）的液相芯片快速检测技术，用DNAStar软件对Gen-Bank中VHSV的G基因进行序列分析，设计VHSV特异性引物并标记生物素。探针氨基化修饰并与荧光编码微球偶联后与VHSV RT-PCR产物杂交反应，用液相芯片仪器检测荧光信号。结果表明液相芯片检测体系能够正确检出VHSV。病毒核酸的最低检出量为10pg，检测特异性高，说明初步建立了检测VHSV的液相芯片技术，为VHSV的检测提供了新方法。
위건립검측병독성출혈성패혈증병독（VHSV）적액상심편쾌속검측기술，용DNAStar연건대Gen-Bank중VHSV적G기인진행서렬분석，설계VHSV특이성인물병표기생물소。탐침안기화수식병여형광편마미구우련후여VHSV RT-PCR산물잡교반응，용액상심편의기검측형광신호。결과표명액상심편검측체계능구정학검출VHSV。병독핵산적최저검출량위10pg，검측특이성고，설명초보건립료검측VHSV적액상심편기술，위VHSV적검측제공료신방법。
In order to develop a liquid chip assay for detection of viral hemorrhagic septicemia of salmonids virus (VHSV),the G gene of VHSV in the GenBank was sequenced by using the software DNAStar 7.0 and specific VHSV primers labeled with biotin were prepared and coupled with fluorescence-coded microspheres. The aminationed probe was used for hybridization of VHSV PCR products,and a liquid chip detection technique for detection of VHSV was established by using BD FACSArray to detect fluorescence signal in the reaction system. The results showed that VHSV could be accurately detected by the developed liquid chip detection technique. The lower detection limit with this method was 10 pg of viral RNA,and the specificity of this method was very high.The study provided a new method for rapid and high throughput detection of VHSV.