医学信息
醫學信息
의학신식
MEDICAL INFORMATION
2015年
24期
62-63
,共2页
莫似恩%朱小凤%梁志锋%唐%林耀旺%杨晓梅%刘林%陆璐%张璇%曾广柳%伍朝强%梁佩艳%唐悦%吴虹欢
莫似恩%硃小鳳%樑誌鋒%唐%林耀旺%楊曉梅%劉林%陸璐%張璇%曾廣柳%伍朝彊%樑珮豔%唐悅%吳虹歡
막사은%주소봉%량지봉%당%림요왕%양효매%류림%륙로%장선%증엄류%오조강%량패염%당열%오홍환
宫内膜容受性%孕激素受体%体外受精-胚胎移植%不孕症%子宫内膜%子宫内膜息肉
宮內膜容受性%孕激素受體%體外受精-胚胎移植%不孕癥%子宮內膜%子宮內膜息肉
궁내막용수성%잉격소수체%체외수정-배태이식%불잉증%자궁내막%자궁내막식육
Receptivity of endometrium%Progestrone receptors(PR)%In Vitro Fertilization-Embryo Transfer(IVF-ET)%Infertility%Endometrium%Endometrial Polyps(EP)
目的通过收集在广西生殖健康中心行体外受精-胚胎移植(IVF-ET)不孕症妇女子宫内膜和息肉组织做病理切片苏木素-伊红(HE)染色和孕激素受体(PR)免疫组化染色，观察PR表达分布情况，为研究提高子宫内膜息肉(EP)不孕症患者妊娠率提供参考依据。方法在广西生殖健康中心收集30例确诊为EP不孕症并行IVF-ET患者的子宫内膜和息肉组织作研究组，另取30例同期在本中心以丈夫不育为主因行IVF-ET的非EP患者(接近正常)子宫内膜组织作正常对照组，同期再在广西医科大学一附院计生门诊收集30例非EP、非不孕症的放取环诊刮患者子宫内膜组织作非IVF-ET对照组，分别进行病理切片HE染色及PR免疫组化染色。结果免疫组化染色显示，正常对照组腺上皮和间质细胞胞浆和胞核均未有明显阳性表达；放取环非IVF-ET组可见腺上皮和间质细胞普遍阳性表达，腺上皮的胞浆、胞核均有表达，间质细胞部分胞核表达；研究组内膜组织主要为腺上皮胞核强阳性表达，腺腔内分泌物及间质细胞也有一定阳性表达；研究组息肉组织主要为局部腺上皮胞浆强阳性表达，胞核少表达，间质细胞轻度阳性表达。结论广西IVE-ET的EP不孕患者子宫内膜组织PR在腺上皮胞核强阳性表达而间质细胞有一定表达，息肉组织局部腺上皮胞浆强阳性表达，胞核少表达，间质细胞轻度阳性表达。
目的通過收集在廣西生殖健康中心行體外受精-胚胎移植(IVF-ET)不孕癥婦女子宮內膜和息肉組織做病理切片囌木素-伊紅(HE)染色和孕激素受體(PR)免疫組化染色，觀察PR錶達分佈情況，為研究提高子宮內膜息肉(EP)不孕癥患者妊娠率提供參攷依據。方法在廣西生殖健康中心收集30例確診為EP不孕癥併行IVF-ET患者的子宮內膜和息肉組織作研究組，另取30例同期在本中心以丈伕不育為主因行IVF-ET的非EP患者(接近正常)子宮內膜組織作正常對照組，同期再在廣西醫科大學一附院計生門診收集30例非EP、非不孕癥的放取環診颳患者子宮內膜組織作非IVF-ET對照組，分彆進行病理切片HE染色及PR免疫組化染色。結果免疫組化染色顯示，正常對照組腺上皮和間質細胞胞漿和胞覈均未有明顯暘性錶達；放取環非IVF-ET組可見腺上皮和間質細胞普遍暘性錶達，腺上皮的胞漿、胞覈均有錶達，間質細胞部分胞覈錶達；研究組內膜組織主要為腺上皮胞覈彊暘性錶達，腺腔內分泌物及間質細胞也有一定暘性錶達；研究組息肉組織主要為跼部腺上皮胞漿彊暘性錶達，胞覈少錶達，間質細胞輕度暘性錶達。結論廣西IVE-ET的EP不孕患者子宮內膜組織PR在腺上皮胞覈彊暘性錶達而間質細胞有一定錶達，息肉組織跼部腺上皮胞漿彊暘性錶達，胞覈少錶達，間質細胞輕度暘性錶達。
목적통과수집재엄서생식건강중심행체외수정-배태이식(IVF-ET)불잉증부녀자궁내막화식육조직주병리절편소목소-이홍(HE)염색화잉격소수체(PR)면역조화염색，관찰PR표체분포정황，위연구제고자궁내막식육(EP)불잉증환자임신솔제공삼고의거。방법재엄서생식건강중심수집30례학진위EP불잉증병행IVF-ET환자적자궁내막화식육조직작연구조，령취30례동기재본중심이장부불육위주인행IVF-ET적비EP환자(접근정상)자궁내막조직작정상대조조，동기재재엄서의과대학일부원계생문진수집30례비EP、비불잉증적방취배진괄환자자궁내막조직작비IVF-ET대조조，분별진행병리절편HE염색급PR면역조화염색。결과면역조화염색현시，정상대조조선상피화간질세포포장화포핵균미유명현양성표체；방취배비IVF-ET조가견선상피화간질세포보편양성표체，선상피적포장、포핵균유표체，간질세포부분포핵표체；연구조내막조직주요위선상피포핵강양성표체，선강내분비물급간질세포야유일정양성표체；연구조식육조직주요위국부선상피포장강양성표체，포핵소표체，간질세포경도양성표체。결론엄서IVE-ET적EP불잉환자자궁내막조직PR재선상피포핵강양성표체이간질세포유일정표체，식육조직국부선상피포장강양성표체，포핵소표체，간질세포경도양성표체。
Objective To observe the expression and distribution of Progestrone Receptors(PR) Immunohistochemical staining in endometrium and Endometrial Polyp(EP) tissues who performed In Vitro Fertilization-Embryo Transfer(IVF-ET) in Guangxi Reproductive Health Center (GXRHC), so that to provide the reference for researching how to improve pregnancy rate in EP patients with infertility. Methods Col ected 30 patients who were diagnosed with EP infertility and planned perform IVF-ET in GXRHC as a research group;meanwhile, the other similar 30 cases were male factor (husband infertility) but nor EP (close to the normal) as a normal control group, 30 cases were col ected in the family planning outpatients of First Af iliated Hospital of Guangxi Medical University who were put in or taken out an intrauterine device (IUD) as a nor IVF-ET control group. Al the endometrium and/or endometrial polyps tissues were obtained by hysteroscopic polypectomy or curet age, and were done HE staining and PR immunohistochemical staining, and then observed the expression of PR. Results Compared with the control group, nuclei of glandular cel s showed higher positive expression,lumens secretions and interstitial cel had a certain positive expression in research group endometrium; but in endometrial polyps, local region cytoplasm of glandular cel s showed strong positive expression, the nuclei of glandular cel s seldom expression, and the interstitial cel to showed a lit le bit expressed. Conclusion Nuclei of glandular cel s showed higher positive expression in endometrium but part region cytoplasm of glandular cel s showed strong positive expression in endometrial polyps of EP infertility women who performed IVE-ET in Guangxi.
