CAJ | 학술논문

目的：通过活性成分的 HPLC 含量测定，为控制桑黄质量提供依据。方法收集市场上常见的桑黄品种样品，用高效液相色谱法测定 hispolon 和原儿茶酸含量。色谱柱为 UItimateAQ －C18（4．6mm ×250mm，5μm）；（A）测定 hi－spolon 流动相为乙腈－水（38：62），检测波长363nm，（B）测定原儿茶酸流动相为乙腈－0．1％磷酸溶液（12：88），检测波长256nm；柱温为25℃；流速为1mL／min；进样量为10μL。结果 hispolon 及原儿茶酸分别在线性范围1．3－0．043mg／mL（r ＝0．9997），0．4－0．025mg／mL（r ＝0．9998）线性关系良好。鲍氏针层孔菌、裂蹄针层孔菌、火木针层孔菌、忍冬木层孔菌不同种、不同宿主的9个样品均含有原儿茶酸，含量42．8－96．8μg／g。松木上生长的不同种桑黄，均未检出 hispolon，其它样品含有 hispolon，含量90．9－223．3μg／g。结论不同桑黄样品均含有原儿茶酸，可作为桑黄的指示成分进行检测。受试样品 hispolon 含量差异大，表明品种、宿主和生长环境对桑黄成分具有明显影响，影响最大的是宿主，松木上生长的桑黄，均未检出 hispolon。监测 hispolon 含量，对控制桑黄质量，具有重要意义。
목적：통과활성성분적 HPLC 함량측정，위공제상황질량제공의거。방법수집시장상상견적상황품충양품，용고효액상색보법측정 hispolon 화원인다산함량。색보주위 UItimateAQ －C18（4．6mm ×250mm，5μm）；（A）측정 hi－spolon 류동상위을정－수（38：62），검측파장363nm，（B）측정원인다산류동상위을정－0．1％린산용액（12：88），검측파장256nm；주온위25℃；류속위1mL／min；진양량위10μL。결과 hispolon 급원인다산분별재선성범위1．3－0．043mg／mL（r ＝0．9997），0．4－0．025mg／mL（r ＝0．9998）선성관계량호。포씨침층공균、렬제침층공균、화목침층공균、인동목층공균불동충、불동숙주적9개양품균함유원인다산，함량42．8－96．8μg／g。송목상생장적불동충상황，균미검출 hispolon，기타양품함유 hispolon，함량90．9－223．3μg／g。결론불동상황양품균함유원인다산，가작위상황적지시성분진행검측。수시양품 hispolon 함량차이대，표명품충、숙주화생장배경대상황성분구유명현영향，영향최대적시숙주，송목상생장적상황，균미검출 hispolon。감측 hispolon 함량，대공제상황질량，구유중요의의。
s:Objective To provide the basis for controlling quality of igniarius phellinus , we determined the active constituent contents by HPLC.Method Collecting the common phellinus igniarius varities as the samples , we analyzed the contents of hispolon and protocate -chuic acid using the HPLC method with UItimateAQ -C18 column (4.6mm ×250mm, 5μm) in 25℃ (column temperature).Hispolon was detected with 38% acetonitrile in water under 363nm ( detecting wavelength ), and protocatechuic acid was 12% acetonitrile in 0.1%psophate solution at 256nm.The injection volum was 10μL with the flow rate of 1mL/min.Result The liner ranges of hispolon and proto -catechuic acid were 1.3 -0.043mg/mL(r =0.9997)and 0.4 -0.025mg/mL(r =0.9998), respectively.The contents of protocatechuic acid in the nine kinds of samples (Phellinus baumii, Phellinus linteus, Phellinus igniariusPhellinus lonicerinu ect .) were from 42.8μg/g to 96.8μg/g.Hisplon was not found in live phellinus igniarius on pine tree , but the contents of other verities were from 90.9μg/g to 223.3μg/g.Conclusion Protocatechuic acid was found in all kinds of phellinus igniarius , which could be determined as an indicating ingredient . The content of hispolon in igniarius phellinus was seriously influenced by the varities , parasitifers and growing environment .Determining the content of hispolon has an important implication for the control the quality of phellinus igniarius .