CAJ | 학술논문

目的：观察疏肝和胃汤对抑郁模型大鼠延髓、脊髓及胃粘膜组织诱导型一氧化氮合酶（i －Nos）表达的影响，探讨疏肝和胃汤改善抑郁状态及胃肠功能的作用机制。方法100只大鼠随机分成生理盐水组、模型组、百优解组和疏肝和胃汤大、小剂量组。采用慢性心理应激加孤养法制作抑郁模型，共计造模4周。在第3周，百优解组及疏肝和胃汤大、小剂量组分别予以0．36mg／（kg? d）、20g／（kg? d）、10g／（kg? d）灌胃给药，生理盐水组及模型组给予等体积生理盐水，每天分2次灌胃。在第4周结束后，使用内固定法取大鼠延髓迷走神经背核部位的脑、胸髓（T6－T8，根据脊神经定位）及胃粘膜组织，应用免疫组织化学技术检测上述部位 i －Nos 的表达。结果模型组大鼠延髓、脊髓及胃黏膜中 i －Nos 阳性表达明显上升，与生理盐水组比较有非常显著性差异（P ＜0．01）；治疗2周后，疏肝和胃汤大、小剂量组和百优解组大鼠延髓、脊髓及胃黏膜组织中 i －Nos 阳性表达显著下降，与模型组比较有显著性差异（P ＜0．05或 P ＜0．01）；疏肝和胃汤大、小剂量组与百优解组比较，除外大剂量组延髓中 i －Nos 含量无显著性差异（P ＞0．05），其余均有显著性差异（P ＜0．05或 P ＜0．01）。结论疏肝和胃汤可能是通过降低延髓、脊髓及胃黏膜中 i －Nos 的表达，调节“脑（延髓）－脊髓－胃”脑肠轴通路中 i －Nos 的含量，达到改善抑郁状态与胃肠功能的作用。
목적：관찰소간화위탕대억욱모형대서연수、척수급위점막조직유도형일양화담합매（i －Nos）표체적영향，탐토소간화위탕개선억욱상태급위장공능적작용궤제。방법100지대서수궤분성생리염수조、모형조、백우해조화소간화위탕대、소제량조。채용만성심리응격가고양법제작억욱모형，공계조모4주。재제3주，백우해조급소간화위탕대、소제량조분별여이0．36mg／（kg? d）、20g／（kg? d）、10g／（kg? d）관위급약，생리염수조급모형조급여등체적생리염수，매천분2차관위。재제4주결속후，사용내고정법취대서연수미주신경배핵부위적뇌、흉수（T6－T8，근거척신경정위）급위점막조직，응용면역조직화학기술검측상술부위 i －Nos 적표체。결과모형조대서연수、척수급위점막중 i －Nos 양성표체명현상승，여생리염수조비교유비상현저성차이（P ＜0．01）；치료2주후，소간화위탕대、소제량조화백우해조대서연수、척수급위점막조직중 i －Nos 양성표체현저하강，여모형조비교유현저성차이（P ＜0．05혹 P ＜0．01）；소간화위탕대、소제량조여백우해조비교，제외대제량조연수중 i －Nos 함량무현저성차이（P ＞0．05），기여균유현저성차이（P ＜0．05혹 P ＜0．01）。결론소간화위탕가능시통과강저연수、척수급위점막중 i －Nos 적표체，조절“뇌（연수）－척수－위”뇌장축통로중 i －Nos 적함량，체도개선억욱상태여위장공능적작용。
Abstracts:Objective To observe the effect of Shuganhewei Decoction (SHD) on the expression of inducible Nitric oxide synthase (i -Nos) in depression model rats of brain(medulla oblongata)、spinal cord and gastric mucosa , to research the possible mechanism of SHD to relieve depression and improve gastrointestinal function .Methods Totally 100 wistar rats were randomized into thenormalsaline group , mod-elgroup, Fluoxetine group and the high, low dosage of SHD groups, with 20 in each.The depression models were established with Chronic Unpredictable Mild Stress (CUMS) and separation according to related references for 4 weeks.At the 3rd week, the model each treatment group began to medicine.Fluoxetine group and high, low dosage of SHD group and were given Fluoxetine and high , low dosage of SHD by 0.36mg/(kg? d)、20g/(kg? d) and 10g/(kg? d) in weights through introgastric administration respectively , twice a day; the normal sa-line group and model group were given the same volume physiological saline .Every group must be given continuous course treatment for four -teen days.At the end of the fourth week, the internal fixation method was used to take brain (medulla oblongata vagus nervenucleus ) tissue、spinal cord tissue (T6 -T8) and gastric mucosa tissue.The method of immunohistochemistry (IHC) was used to detect the expression of i -Nos in the above organizations.Results Comparing with the normal saline group , the content of i -Nos increased obviously in brain (me-dulla oblongata) 、spinal cord and gastric mucosa of model rats (P <0.01).Comparing with the model group , the content of i -Nos decreased significantly in brain (medulla oblongata) 、spinal cord and gastric mucosa of high , low dosage of SHD group and Fluoxetine group (P <0.05 or P <0.01).In addition, the effect of high dosage of SHD was equal to Fluoxetine group in medulla oblongata (P >0.05), while the former was better than the later in spinal cord and gastric mucosa (P <0.05 or P <0.01).Conclusion SHD had regulation effects on the expres -sion of i -Nos in the signal path of brain -gut axis -“brain(medulla oblongata) -spinal cord -gastric mucosa”, which may be part of the mechanism for relieving depression and improving gastrointestinal function .