浙江临床医学
浙江臨床醫學
절강림상의학
ZHEJIANG CLINICAL MEDICAL JOURNAL
2015年
6期
903-905
,共3页
OXA-23酶%碳青霉烯类%耐药传播%鲍曼不动杆菌
OXA-23酶%碳青黴烯類%耐藥傳播%鮑曼不動桿菌
OXA-23매%탄청매희류%내약전파%포만불동간균
OXA-23 type carbapenemase%Carbapenems%Resistant transmission%A. baumannⅡ
目的:探讨OXA-23酶在介导鲍曼不动杆菌碳青霉烯类抗生素耐药过程中的传播机制。方法对2010年9月至2012年3月58株产OXA-23型碳青霉烯酶鲍曼不动杆菌进行深入研究,所有菌株运用PCR mapping方法对blaOXA-23基因的周围结构进行扩增及测序分析;根据PFGE型别挑选不同克隆的菌株,进一步采用S1-PFGE以及ApaI-PFGE联合Southern blot杂交法对blaOXA-23基因进行定位分析。结果58株菌中有47株菌的blaOXA-23基因周围结构为Tn2009转座结构、11株为Tn2008转座结构;58株菌属于8个不同的克隆型别(A、B、C、D、E、F1、F2、G和H克隆),其中E克隆的blaOXA-23基因存在2个拷贝,分别位于质粒及染色体上,质粒大小为78.2kb左右;其余克隆菌blaOXA-23基因定位于染色体上,所在片段大小为310.1kb左右,其中D克隆存在2个染色体拷贝。结论转座结构是导致blaOXA-23基因迅速在鲍曼不动杆菌中转移的重要元件,导致鲍曼不动杆菌碳青霉烯类抗生素耐药传播和扩散。
目的:探討OXA-23酶在介導鮑曼不動桿菌碳青黴烯類抗生素耐藥過程中的傳播機製。方法對2010年9月至2012年3月58株產OXA-23型碳青黴烯酶鮑曼不動桿菌進行深入研究,所有菌株運用PCR mapping方法對blaOXA-23基因的週圍結構進行擴增及測序分析;根據PFGE型彆挑選不同剋隆的菌株,進一步採用S1-PFGE以及ApaI-PFGE聯閤Southern blot雜交法對blaOXA-23基因進行定位分析。結果58株菌中有47株菌的blaOXA-23基因週圍結構為Tn2009轉座結構、11株為Tn2008轉座結構;58株菌屬于8箇不同的剋隆型彆(A、B、C、D、E、F1、F2、G和H剋隆),其中E剋隆的blaOXA-23基因存在2箇拷貝,分彆位于質粒及染色體上,質粒大小為78.2kb左右;其餘剋隆菌blaOXA-23基因定位于染色體上,所在片段大小為310.1kb左右,其中D剋隆存在2箇染色體拷貝。結論轉座結構是導緻blaOXA-23基因迅速在鮑曼不動桿菌中轉移的重要元件,導緻鮑曼不動桿菌碳青黴烯類抗生素耐藥傳播和擴散。
목적:탐토OXA-23매재개도포만불동간균탄청매희류항생소내약과정중적전파궤제。방법대2010년9월지2012년3월58주산OXA-23형탄청매희매포만불동간균진행심입연구,소유균주운용PCR mapping방법대blaOXA-23기인적주위결구진행확증급측서분석;근거PFGE형별도선불동극륭적균주,진일보채용S1-PFGE이급ApaI-PFGE연합Southern blot잡교법대blaOXA-23기인진행정위분석。결과58주균중유47주균적blaOXA-23기인주위결구위Tn2009전좌결구、11주위Tn2008전좌결구;58주균속우8개불동적극륭형별(A、B、C、D、E、F1、F2、G화H극륭),기중E극륭적blaOXA-23기인존재2개고패,분별위우질립급염색체상,질립대소위78.2kb좌우;기여극륭균blaOXA-23기인정위우염색체상,소재편단대소위310.1kb좌우,기중D극륭존재2개염색체고패。결론전좌결구시도치blaOXA-23기인신속재포만불동간균중전이적중요원건,도치포만불동간균탄청매희류항생소내약전파화확산。
Objective To illustrate the molecular transmission mechanism of carbapenem-resistance mediated by OXA-23 type carbapenemase in A. baumannⅡ. Methods Fifty-eight strains collected from September 2010 to March 2012 were involved in our study. PCR mapping method followed by sequencing was used to analysis the surrounding structure of the blaOXA-23 gene. Those isolates originating from different clones according to PFGE analysis were picked out to detect the gene location of the blaOXA-23 with S1-PFGE and ApaI-PFGE followed by Southern blotting method. Results 47 in 58 strains harbored the blaOXA-23 gene in Tn2009 transposon structure,while 11 in Tn2008. Only one type of PFGE clone strain (Clone E) harbors the blaOXA-23 gene both in plasmid (ranging around 78.2kb) and in chromosome. The blaOXA-23 gene was located in chromosome in the rest types of clones, in which two copies of clones were found in Clone D. Conclusion Both transposon was the main factors mediated the blaOXA-23 gene transmission among A. baumannⅡ,which was also the molecular basis leading to the epidemic of carbapenem-resistant A. baumannⅡ.