CAJ | 학술논문

利用复苏可培养化VBNC（ Viable but non－culturable bacteria）资源菌筛选了对毒死蜱有降解性能的菌种及其酶．应用最大或然数计数（ MPN）法，添加复苏促进因子Rpf蛋白分子，获取可培养化VBNC菌种，确定菌种的系统进化属性，并用常规培养法筛选了高效毒死蜱降解菌及其酶，探索了适宜的酶降解优化条件．从分离到的可培养化VBNC菌株中筛选出一株降解毒死蜱效果达70％的菌株CHZYR63，经16S rRNA基因序列分析，初步鉴定为荧光假单胞菌（Pseudomonas fluorescens）．继而，从该菌株提取出粗酶，其降解毒死蜱能力为50％．降解条件优化研究表明：降解酶促反应的最适温度为35℃，最适pH值为7；在最适条件下该降解酶在30 min内对毒死蜱（100 mg／L）的降解率可达60．26％．
이용복소가배양화VBNC（ Viable but non－culturable bacteria）자원균사선료대독사비유강해성능적균충급기매．응용최대혹연수계수（ MPN）법，첨가복소촉진인자Rpf단백분자，획취가배양화VBNC균충，학정균충적계통진화속성，병용상규배양법사선료고효독사비강해균급기매，탐색료괄의적매강해우화조건．종분리도적가배양화VBNC균주중사선출일주강해독사비효과체70％적균주CHZYR63，경16S rRNA기인서렬분석，초보감정위형광가단포균（Pseudomonas fluorescens）．계이，종해균주제취출조매，기강해독사비능력위50％．강해조건우화연구표명：강해매촉반응적최괄온도위35℃，최괄pH치위7；재최괄조건하해강해매재30 min내대독사비（100 mg／L）적강해솔가체60．26％．
Using MPN system, some strains of the VBNC ( viable but non-culturable) bacteria were isolated by Rpf ( resuscitation promoting factor) , identifying phylogenetic analysis.The Screening of chlorpyrifos-de-grading bacteria and corresponding enzymes used routine methods, explored the appropriate optimal conditions of the degradation enzyme.One strain—CHZYR63 was selected from the bacteria which could degrade Chlor-pyrifos.Its degradation efficiency could reach 70%.The strain was identified as Pseudomonas fluorescens based on 16S rRNA sequence analysis.Degradation enzyme, whose degradation rate was 50%,was also ex-tracted.A series of studies were conducted to determine its separation conditions and the characteristics of chlorpyrifos degradation.Studies showed that the optimal temperature and pH of enzymatic reaction was 35℃and 7, respectively.Under optimal conditions, its degradation rate of chlorpyrifos (100 mg/L) was as high as 60.26%within 30 minutes.