CAJ | 학술논문

以转AtCBL1、AtCIPK23基因和非转基因甘蔗品种为材料，研究了转AtCBL9和AtCIPK23基因甘蔗在低钾胁迫下(100μmol/L)的生长及生理特征。结果表明，在低供钾胁迫60天后，转 AtCBL9和 AtCIPK23基因甘蔗株高、干物质积累和根系钾含量高于非转基因甘蔗；甘蔗根系活力上升，转基因甘蔗根系活力上升幅度高于非转基因甘蔗；非转基因甘蔗可溶性糖、丙二醛含量、和SOD酶下降，而2个转基因甘蔗中则表现为上升，或下降幅度降低。在甘蔗中过量表达拟南芥 AtCBL9和 AtCIPK23基因有利于甘蔗生长和对低钾的抗性。
이전AtCBL1、AtCIPK23기인화비전기인감자품충위재료，연구료전AtCBL9화AtCIPK23기인감자재저갑협박하(100μmol/L)적생장급생리특정。결과표명，재저공갑협박60천후，전 AtCBL9화 AtCIPK23기인감자주고、간물질적루화근계갑함량고우비전기인감자；감자근계활력상승，전기인감자근계활력상승폭도고우비전기인감자；비전기인감자가용성당、병이철함량、화SOD매하강，이2개전기인감자중칙표현위상승，혹하강폭도강저。재감자중과량표체의남개 AtCBL9화 AtCIPK23기인유리우감자생장화대저갑적항성。
The characteristics of dry matter, potassium accumulation and the physiological differences were investigated in the roots among AtCBL9 and AtCIPK23 transgenic lines and wild type YT00-236 under low potassium stress. The results showed that the transgenic line displayed higher height, dry matter accumulation, and potassium content in roots than that of the wild line under the condition of low potassium supplying. Root system vigor of sugarcane increased in response to low K stress, but the increase ratios of two transgenic lines were higher than that of non-transgenic line. The soluble sugar, MDA content and activities of superoxide dismutase (SOD) in non-transgenic line decreased under low K+ stress. On contrary, they increased in two transgenic lines. In conclude, ectopinc expression of AtCBL9 and AtCIPK23 in sugarcane would improve the growth of sugarcane and increased the tolerance to low potassium condition.