CAJ | 학술논문

目的：探讨波生坦对肺纤维化大鼠肺胶原沉积与MMP－9、TIMP－1的影响。方法将48只成年雄性Wistar大鼠随机均分为6组：对照2周组、4周组（ C2组、C4组），模型2周组、4周组（ M2组、M4组）及治疗组（ T1组、T2组）。予博莱霉素建模， T1、T2组分别于第2 d、第15 d始每天予波生坦灌胃至第28 d。Masson 染色观察肺胶原沉积情况，用酶联免疫反应法测定肺泡灌洗液的MMP－9、TIMP －1浓度。结果（1）C2组、C4组大鼠肺间质胶原稀少；M2组可见大量淡蓝色胶原沉积，M 4组明显加重伴部分纤维化；T1组较 M4组显著减少，T 2组较 M4组无差异。（2）造模后MMP－9浓度随时间升高：C2组、C4组、M2组、M4组分别为（1．15±0．27）μg／mL、（1．23±0．31）μg／mL、（1．98±0．35）μg／mL、（2．38±0．47）μg／mL，T1组较M4组显著下降（P＜0．05）。（3） M2组、M4组TIMP－1浓度明显高于C2组、C4组，T1组较M4组明显下降，差异有统计学意义。结论波生坦可减少肺纤维化大鼠肺组织的胶原沉积，可能是通过早期抑制MMP－9、TIMP－1途径起作用。
목적：탐토파생탄대폐섬유화대서폐효원침적여MMP－9、TIMP－1적영향。방법장48지성년웅성Wistar대서수궤균분위6조：대조2주조、4주조（ C2조、C4조），모형2주조、4주조（ M2조、M4조）급치료조（ T1조、T2조）。여박래매소건모， T1、T2조분별우제2 d、제15 d시매천여파생탄관위지제28 d。Masson 염색관찰폐효원침적정황，용매련면역반응법측정폐포관세액적MMP－9、TIMP －1농도。결과（1）C2조、C4조대서폐간질효원희소；M2조가견대량담람색효원침적，M 4조명현가중반부분섬유화；T1조교 M4조현저감소，T 2조교 M4조무차이。（2）조모후MMP－9농도수시간승고：C2조、C4조、M2조、M4조분별위（1．15±0．27）μg／mL、（1．23±0．31）μg／mL、（1．98±0．35）μg／mL、（2．38±0．47）μg／mL，T1조교M4조현저하강（P＜0．05）。（3） M2조、M4조TIMP－1농도명현고우C2조、C4조，T1조교M4조명현하강，차이유통계학의의。결론파생탄가감소폐섬유화대서폐조직적효원침적，가능시통과조기억제MMP－9、TIMP－1도경기작용。
Objective To observe the effect of bosentan on collagen deposition and MMP -9/TIMP-1 of pulmonary fibrosis (PF). Methods 48maleadultWistarratswererandomlyandequallydividedintocontrolgroups(C2,C4group),modelgroups(M2,M4group) and treated groups(T1, T2 group).Bleomycin was used to create PF models.The collagen deposition in lung was observed by Masson′s tri-chrome stain, and the level of MMP-9 and TIMP-1 in BALF were measured by ELISA.Results (1)There was less collagen deposition in C2 and C4 groups.Light blue collagen deposition could be seen in M 2 group.M4 group showed significant serious fibrosis .T1 group was significantly less than M4 group, and there was no significant difference between T 2 and M4.(2)MMP-9 expression was increased in C2, C4, M2 and M4 groups [(1.15 ±0.27),(1.23 ±0.31), (1.98 ±0.35)、(2.38 ±0.47) ng/mL] , which were significantly lower than in T1 group than M4.The level of TIMP-1 was higher in M2 and M4 groups(P<0.05).(3) TIMP-1 in M2 and M4 groups was signifi-cantly higher than C2, C4 and T1 groups, and significantly lower than M4 group.There was statistical significance.Conclusion Bosentan can alleviate the collagen deposition in lung of pulmonary fibrosis in rats , and inhibit the expressions of MMP -9/TIMP-1 in early stage.