CAJ | 학술논문

目的：通过研究薄荷药材特征图谱与其抗氧化活性的相关关系,探讨薄荷抗氧化活性物质基础。方法采用1,1-二苯基-2-三硝基苯肼(DPPH)、总抗氧化能力评估法(FRAP)法分别测定薄荷样品抗氧化活性,同时测定样品相应的特征图谱,通过偏最小二乘法研究特征图谱色谱峰和抗氧化活性相关性,筛选活性色谱峰,并结合HPLC-MS/MS n 进行结构鉴定。结果筛选得到的橙皮苷、香叶木苷、蒙花苷、百里香新、黄姜味草酸等6个化合物与清除DPPH自由基能力相关,橙皮苷、蒙花苷、反式丹酚酸J、迷迭香酸等8个化合物与总抗氧化能力相关。结论谱效关系研究能够表征中药化学成分-药效相关关系,可以较为快捷地从复杂体系中筛选活性化合物。
목적：통과연구박하약재특정도보여기항양화활성적상관관계,탐토박하항양화활성물질기출。방법채용1,1-이분기-2-삼초기분정(DPPH)、총항양화능력평고법(FRAP)법분별측정박하양품항양화활성,동시측정양품상응적특정도보,통과편최소이승법연구특정도보색보봉화항양화활성상관성,사선활성색보봉,병결합HPLC-MS/MS n 진행결구감정。결과사선득도적등피감、향협목감、몽화감、백리향신、황강미초산등6개화합물여청제DPPH자유기능력상관,등피감、몽화감、반식단분산J、미질향산등8개화합물여총항양화능력상관。결론보효관계연구능구표정중약화학성분-약효상관관계,가이교위쾌첩지종복잡체계중사선활성화합물。
Objective To explore the active ingredients of Menthae Haplocalycis Herba by investigating the correlation between HPLC spectrum and antioxidant activity. Methods We established DPPH[1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl,DPPH] and FRAP (ferric ion reducing antioxidant power, FRAP) assay to determine the antioxidant activity of Menthae Haplocalycis Herba. The spectrum-effect correlation was studied by using PLS(Partial least squares). HPLC-MS/MSn was applied to identify their structures. Results Six compounds including hesperidin, diosmin, buddleoside, thymusin and xanthomicrol, which made great contributions to the DPPH scavenging activity, were screened; eight compounds, including hesperidin, buddleoside, rosmarinic acid and trans Salvianolic acid J, were obtained which made great contributions to the total antioxidant activity. Conclusion It is an effective way to characterize spectrum-efficacy correlation and clarify the active components of Chinese medicinal.