CAJ | 학술논문

目的：观察不同刺激量针刺三阴交对寒凝证类痛经大鼠子宫张力及内分泌相关分子受体基因表达的影响，探寻不同针刺刺激量与靶器官效应之间的关系。方法将动情期间雌性大鼠随机分为盐水组、寒凝证类痛经大鼠模型(模型组)、期望“得气”组(粗针深刺行针的治疗方法)、期望“不得气”组(细针浅刺不行针的治疗方法)；期望“得气”组或期望“不得气”组均在成功制备模型基础上给予治疗，并于针刺进行时观察20 min内在体大鼠子宫收缩强度及大鼠子宫组织前列腺素EP2受体mRNA (PGEP2mRNA)、前列腺素F2α受体mRNA(PGF2αmRNA)表达量。结果子宫收缩强度检测方面，与模型组比较，期望“得气”组显著减少了子宫收缩波个数(P<0.01)，显著降低子宫活动度(P<0.05)。子宫PGEP2 mRNA及PGF2αmRNA检测方面，与模型组比较，期望“得气”组及期望“不得气”组 PGF2αmRNA表达显著降低( P<0.01)。结论不同刺激量针刺三阴交穴均能缓解寒凝证类痛经大鼠子宫收缩强度及降低子宫内分泌相关分子受体基因表达，使用粗针深刺加行针的治疗效果优于细针浅刺不行针，认为粗针深刺加行针的刺激量更能达到针灸“得气”的状态。
목적：관찰불동자격량침자삼음교대한응증류통경대서자궁장력급내분비상관분자수체기인표체적영향，탐심불동침자자격량여파기관효응지간적관계。방법장동정기간자성대서수궤분위염수조、한응증류통경대서모형(모형조)、기망“득기”조(조침심자행침적치료방법)、기망“불득기”조(세침천자불행침적치료방법)；기망“득기”조혹기망“불득기”조균재성공제비모형기출상급여치료，병우침자진행시관찰20 min내재체대서자궁수축강도급대서자궁조직전렬선소EP2수체mRNA (PGEP2mRNA)、전렬선소F2α수체mRNA(PGF2αmRNA)표체량。결과자궁수축강도검측방면，여모형조비교，기망“득기”조현저감소료자궁수축파개수(P<0.01)，현저강저자궁활동도(P<0.05)。자궁PGEP2 mRNA급PGF2αmRNA검측방면，여모형조비교，기망“득기”조급기망“불득기”조 PGF2αmRNA표체현저강저( P<0.01)。결론불동자격량침자삼음교혈균능완해한응증류통경대서자궁수축강도급강저자궁내분비상관분자수체기인표체，사용조침심자가행침적치료효과우우세침천자불행침，인위조침심자가행침적자격량경능체도침구“득기”적상태。
Objective To evaluate the effects of acupuncture with different stimulation intensity at Sanyinjiao (SP6) on uterine contraction and expression of endocrine-related molecular receptor gene in rats with dysmenorrhea of cold coagulation pattern and to explore whether there is association between“deqi ( qi arrival)” and stimulus intensity. Methods Diestrus female rats were randomly divided into 4 groups, in-cluding saline control group, model control group, deqi group ( with strong stimulus) and No deqi group ( with weak stimulus) . The levels of uterine contraction was recorded during 20 mins acupuncture treat- <br> ment. The expression of PGF2α mRNA was measured. Results Compared with the model control group, the uterine contraction wave number and uterine activity were significantly reduced in deqi group. The ex-pressions of PGF2αmRNA in both deqi and No deqi group were also reduced compared with model group. Conclusion Our finding indicates that using thick needles, deep insertion with hand-manipulation seems more effective and could achieve desired level of “deqi” in dysmenorrhea rats.