CAJ | 학술논문

目的：比较截断疗法和常规疗法对小鼠流感病毒性肺炎的治疗作用。方法 ICR小鼠80只,分为正常组、模型组、常规疗法组、截断疗法组,以流感病毒鼠肺适应株FM1病毒液滴鼻感染后1h灌胃给药,截断疗法组以犀角地黄汤合银翘散水煎剂灌胃；常规疗法组第1、2、3天以银翘散水煎剂灌胃,第4、5、6、7天以犀角地黄汤水煎剂灌胃,连续给药7 d,观察14 d,计算小鼠的生存率、平均存活天数、体重变化。 Balb/c 小鼠128只,分为以上4组,处理同上,分别于第2、4、6、8天处死动物并取材、检测。观察不同时间点小鼠肺组织病毒滴度、肺指数；观察第8天小鼠肺大体及肺组织病理变化。结果截断疗法组与常规疗法组比较,生存率升高一倍,平均存活天数明显升高；截断疗法组在第6、8天较常规疗法组肺指数明显降低,在第2、4、6、8天肺组织病毒滴度较常规疗法组均有所下降,但无统计学意义。结合肉眼观察肺大体病变及光镜下观察小鼠肺部病理改变,截断疗法组病变较常规疗法组显著减轻。结论截断疗法对重症小鼠流感病毒性肺炎的治疗作用优于常规疗法,其机制可能不在于对病毒的抑制作用强,而在于对病毒感染后机体发生的炎症级联反应抑制作用强。
목적：비교절단요법화상규요법대소서류감병독성폐염적치료작용。방법 ICR소서80지,분위정상조、모형조、상규요법조、절단요법조,이류감병독서폐괄응주FM1병독액적비감염후1h관위급약,절단요법조이서각지황탕합은교산수전제관위；상규요법조제1、2、3천이은교산수전제관위,제4、5、6、7천이서각지황탕수전제관위,련속급약7 d,관찰14 d,계산소서적생존솔、평균존활천수、체중변화。 Balb/c 소서128지,분위이상4조,처리동상,분별우제2、4、6、8천처사동물병취재、검측。관찰불동시간점소서폐조직병독적도、폐지수；관찰제8천소서폐대체급폐조직병리변화。결과절단요법조여상규요법조비교,생존솔승고일배,평균존활천수명현승고；절단요법조재제6、8천교상규요법조폐지수명현강저,재제2、4、6、8천폐조직병독적도교상규요법조균유소하강,단무통계학의의。결합육안관찰폐대체병변급광경하관찰소서폐부병리개변,절단요법조병변교상규요법조현저감경。결론절단요법대중증소서류감병독성폐염적치료작용우우상규요법,기궤제가능불재우대병독적억제작용강,이재우대병독감염후궤체발생적염증급련반응억제작용강。
Objective To compare the effects of truncated and routine methods in the treatment of mice pneumonia induced by influenza virus. Methods ICR mice (n=80) were randomly divided into normal group, model group, routine treatment group and truncated treatment group. The last three groups were infected with mouse lung-adapted influenza virus strain by inoculating intranasally. One hour after the inoculation, the truncated treatment group was intragastrically given Xijiao dihuang Tang ( Rhinoceros Horn and Rehmannia Decoction) combined with Yinqiao San ( Lonicera and Forsythia Powder) decoction while the routine treatment group was intragastrically given Yinqiao San decoction only on the first three days and Xijiao dihuang Tang only on the following four days. All groups were treated for 7 consecutive days. The survival rate, average survival days and body weights were calculated during 14 days ’ observation. Balb/c mice ( n =128 ) were divided into the same four groups described above with the same corresponding intervention. These mice were sacrificed on the 2nd, 4th, 6th and 8th days respective-ly. Specimen were obtained and evaluated. The viral titers in lung homogenate and the lung index were <br> measured dynamically;the lung gross lesions and tissue pathological changes were observed on the 8 th day. Results The survival rate of truncated treatment group was twice as high as routine treatment group and average survival days also increased significantly; the lung index of truncated therapy group significantly decreased compared with the routine group on the 8th day. The viral titers of truncated treatment group in lung homogenate decreased on the 2 nd , 4 th , 6 th and 8 th day compared with the routine treatment group, yet the difference was not statistically significant. The pulmonary gross lesions and tissue pathological changes under light microscope were less severe than the routine treatment group. Conclusion Truncated method seemed to be superior to routine method in the treatment of severe influenza viral pneumonia mice. Its mechanisms may not lie in its stronger anti-viral effect, but in its stronger inhibitory effect on inflammatory cascade after viral infection compared with routine treatment.