CAJ | 학술논문

目的：研究疏肝清热健脾法对广泛性焦虑( GAD)大鼠脑内Papez环路区域的B细胞白血病/淋巴瘤-2(bcl-2)、B细胞白血病/淋巴瘤-2相关蛋白(bax)2种蛋白表达的影响。方法将40只雄性Wistar大鼠随机分为正常组、模型组、中药治疗组和西药对照组4组,每组10只大鼠。模型组、治疗组、对照组采用不确定空瓶饮水刺激方法制备广泛性焦虑大鼠模型。治疗组和对照组分别以丹栀逍遥散(10.5 g/kg)和盐酸丁螺环酮(2 mg/kg)水溶液灌胃7 d,每天1次。模型组蒸馏水灌胃7 d。取脑组织,western blot法检测大鼠脑内海马和乳头体组织bax和bcl-2的含量。结果与正常组比较,模型组海马和乳头体组织的bax含量显著增加,差异有统计学意义( P <0.05或P <0．01),bcl-2的含量有较明显的下降,差异有统计学意义(P<0.01)；与模型组相比,治疗组海马及乳头体组织的bax表达均有降低,差异有统计学意义( P<0.05或P<0.01), bcl-2的表达均有增加,差异有统计学意义( P<0.01)。结论以丹栀逍遥散为代表的疏肝清热健脾法能够降低GAD大鼠脑内海马和乳头体组织bax的水平,升高bcl-2的水平,降低bax/bcl-2值,干预和抑制Papez环路区域的细胞凋亡,从而改善焦虑症状。
목적：연구소간청열건비법대엄범성초필( GAD)대서뇌내Papez배로구역적B세포백혈병/림파류-2(bcl-2)、B세포백혈병/림파류-2상관단백(bax)2충단백표체적영향。방법장40지웅성Wistar대서수궤분위정상조、모형조、중약치료조화서약대조조4조,매조10지대서。모형조、치료조、대조조채용불학정공병음수자격방법제비엄범성초필대서모형。치료조화대조조분별이단치소요산(10.5 g/kg)화염산정라배동(2 mg/kg)수용액관위7 d,매천1차。모형조증류수관위7 d。취뇌조직,western blot법검측대서뇌내해마화유두체조직bax화bcl-2적함량。결과여정상조비교,모형조해마화유두체조직적bax함량현저증가,차이유통계학의의( P <0.05혹P <0．01),bcl-2적함량유교명현적하강,차이유통계학의의(P<0.01)；여모형조상비,치료조해마급유두체조직적bax표체균유강저,차이유통계학의의( P<0.05혹P<0.01), bcl-2적표체균유증가,차이유통계학의의( P<0.01)。결론이단치소요산위대표적소간청열건비법능구강저GAD대서뇌내해마화유두체조직bax적수평,승고bcl-2적수평,강저bax/bcl-2치,간예화억제Papez배로구역적세포조망,종이개선초필증상。
Objective To explore the effects of liver-soothing heat-clearing and spleen-fortifying method on the expressions of bax and bcl-2 in cerebral Papez’ s circuit in rat with generalized Anxiety Disease ( GAD) . Methods A total of 40 male Wistar rats were divided into normal group, model group, treat-ment group, and control group, with 10 rats in each group. GAD model was established by using the stimulation of uncertain empty drinking bottle. Then the treatment group and control group were intragas-trically given Danzhi Xiaoyao San ( Cortex Moutan and Fructus Gardeniae Free Wanderer Powder ) and Buspirone Hydrochloride once a day for 7 consecutive days respectively. The model group was given dis-tilled water once a day for a week. The expressions of bax and bcl-2 in rat hippocampus and mamillary <br> body were measured by applying western blot assay after resecting rat brains. Results The expression of bax in hippocampus and mamillary body of model group increased significantly compared with normal group ( P<0 . 05 or P<0 . 01 ) and the expression of bcl-2 decreased markedly ( P<0 . 01 ) . Compared with model group, the level of bax in hippocampus and mamillary body of treatment group and control group was reduced ( P<0 . 05 or P<0 . 01 ) and the level of bcl-2 increased significantly ( P<0 . 05 or P<0 . 01 ) . Conclusion The therapeutic method of liver-soothing heat-clearing and spleen-fortifying method represented by Danzhi Xiaoyao San could reduce the level of bax in hippocampus and mamillary body of GAD rat brain, elevate the level of bcl-2 and reduce bax/bcl-2, prevent and inhibit cell apoptosis in Papez’ s circuit and then ameliorate anxiety symptoms.