CAJ | 학술논문

目的：探讨熟地黄多糖体内诱导肿瘤细胞凋亡的作用机理，观察熟地黄多糖对肿瘤细胞中Bcl－2蛋白表达的影响及细胞凋亡形态学上的变化，为临床应用提供实验依据。方法：采用H22肝癌腹水瘤株接种小鼠制备模型，随机分为模型组、阳性对照组、熟地黄多糖组。免疫组化法检测肿瘤组织中Bcl－2蛋白表达、TUNEL法检测细胞凋亡指数。结果：与模型组比较，熟地黄多糖组的Bcl－2蛋白平均光密度值降低，与模型组比较差异显著（P＜0．01）；熟地黄多糖组的凋亡指数明显上升（P＜0．05）。结论：熟地黄多糖对H22荷瘤小鼠肿瘤组织中Bcl－2蛋白的表达有明显的抑制作用，使凋亡细胞数目明显增多，熟地黄多糖能有效诱导H22荷瘤小鼠肿瘤细胞发生凋亡。
목적：탐토숙지황다당체내유도종류세포조망적작용궤리，관찰숙지황다당대종류세포중Bcl－2단백표체적영향급세포조망형태학상적변화，위림상응용제공실험의거。방법：채용H22간암복수류주접충소서제비모형，수궤분위모형조、양성대조조、숙지황다당조。면역조화법검측종류조직중Bcl－2단백표체、TUNEL법검측세포조망지수。결과：여모형조비교，숙지황다당조적Bcl－2단백평균광밀도치강저，여모형조비교차이현저（P＜0．01）；숙지황다당조적조망지수명현상승（P＜0．05）。결론：숙지황다당대H22하류소서종류조직중Bcl－2단백적표체유명현적억제작용，사조망세포수목명현증다，숙지황다당능유효유도H22하류소서종류세포발생조망。
Objective:To investigate RPS vivo anti-tumor mechanism of induction of apoptosis of tumor cells, the impact of Bcl-2 protein expression and apoptotic morphological changes,and to provide the basis for clini-cal application.Methods:The H22 ascites mice were inoculated with tumor strain, and the preparation models were divided into the saline group,the cyclophosphamide group and the RPS group.Immunohistochemistry was used to detect tumor tissue Bcl-2 protein expression and apoptosis by TUNEL index.Results:Compared with the model group,Bcl-2 protein mean optical density in the RPS group was lower,and the difference was sig-nificant(P<0.01).The apoptotic index in the RPS group increased significantly(P<0.05).Conclusion:RPS can inhibit the H22 tumor tissue expression of Bcl-2 in mice,so the number of apoptotic cells increases signifi-cantly,and RPS can effectively induce apoptosis in tumor-bearing mice.