中医药信息
中醫藥信息
중의약신식
INFORMATION ON TRADITIONAL CHINESE MEDICINE
2015年
4期
25-28
,共4页
孟永海%史连宏%王欣慰%盖应丽%翟春梅
孟永海%史連宏%王訢慰%蓋應麗%翟春梅
맹영해%사련굉%왕흔위%개응려%적춘매
石膏%抗体夹心酶联免疫吸附法%CYP450酶%CYP1A2%CYP2E1
石膏%抗體夾心酶聯免疫吸附法%CYP450酶%CYP1A2%CYP2E1
석고%항체협심매련면역흡부법%CYP450매%CYP1A2%CYP2E1
Gypsum%Antibody sandwich enzyme-linked immunosorbent method%CYP450 enzyme%CYP1A2%CYP2 E1
目的：研究石膏水煎液对肝微粒体细胞色素P450酶CYP1A2和CYP2E1活性的影响，指导临床合理用药。方法：采用双抗体夹心酶联免疫吸附试验（ ELISA）对肝脏细胞色素P450酶CYP1A2和CYP2 E1进行了初步研究。结果：与空白对照组比较，石膏水煎液对大鼠肝脏中的CYP1 A2含量有降低的趋势，对CYP2 E1的含量没有显著影响。结论：石膏对大鼠肝微粒体CYP1 A2活性有抑制作用，临床上当与经过CYP1 A2代谢的药物合用时应适当调整药量。
目的：研究石膏水煎液對肝微粒體細胞色素P450酶CYP1A2和CYP2E1活性的影響，指導臨床閤理用藥。方法：採用雙抗體夾心酶聯免疫吸附試驗（ ELISA）對肝髒細胞色素P450酶CYP1A2和CYP2 E1進行瞭初步研究。結果：與空白對照組比較，石膏水煎液對大鼠肝髒中的CYP1 A2含量有降低的趨勢，對CYP2 E1的含量沒有顯著影響。結論：石膏對大鼠肝微粒體CYP1 A2活性有抑製作用，臨床上噹與經過CYP1 A2代謝的藥物閤用時應適噹調整藥量。
목적：연구석고수전액대간미립체세포색소P450매CYP1A2화CYP2E1활성적영향，지도림상합리용약。방법：채용쌍항체협심매련면역흡부시험（ ELISA）대간장세포색소P450매CYP1A2화CYP2 E1진행료초보연구。결과：여공백대조조비교，석고수전액대대서간장중적CYP1 A2함량유강저적추세，대CYP2 E1적함량몰유현저영향。결론：석고대대서간미립체CYP1 A2활성유억제작용，림상상당여경과CYP1 A2대사적약물합용시응괄당조정약량。
Objective: To study the influence of Gypsum water decoction on the activity of CYP1 A2 and CYP2E1 of the liver microsomal cytochrome P450,and to guide clinically rational medicine use.Methods:Use a preliminary study with double antibody sandwich enzyme -linked immunosorbent assay ( ELISA ) for CYP1A2 and CYP2E1 of cytochrome P450.Results: Compared with the blank control group, the effect of Gypsum water decoction on the content of CY1A2 in the liver of rats had a reducced tendency,but it had no significant influence on the content of CYP2E1.Conclusion: Gypsum water decoction has an inhibitory effect on the activity of CYP1 A2 , and doctors should adjust the dosage when it is used with medicine which is after CYP1A2 metabolism at the same time.
