CAJ | 학술논문

不同针刺刺激量对寒凝证类痛经模型大鼠扭体反应及子宫内前列腺素物质含量的影响
불동침자자격량대한응증류통경모형대서뉴체반응급자궁내전렬선소물질함량적영향
Effect of Different Acupuncture Stimulus on Writhing Response and Prostaglandin Content of Dysmenorrhea Rats with Cold Syndrome

万方数据

中医药信息中醫藥信息 중의약신식
INFORMATION ON TRADITIONAL CHINESE MEDICINE
2015年 4期 48-51 ,共4页

齐丹丹%张玲%申松希%朱世鹏%沈小雨%许咏思%杨佳敏%罗丽%任晓暄%郭孟玮%赵雅芳%李晓泓%嵇波%张露芬%朱江

제단단%장령%신송희%주세붕%침소우%허영사%양가민%라려%임효훤%곽맹위%조아방%리효홍%혜파%장로분%주강

痛经%针刺刺激量%得气%扭体评分%PGF2α%PGE2 痛經%針刺刺激量%得氣%扭體評分%PGF2α%PGE2
통경%침자자격량%득기%뉴체평분%PGF2α%PGE2
Dysmenorrhea%Stimulus of needling%Deqi%Writhing score%PGF2α%PGE2

目的：观察不同针刺刺激量干预三阴交穴后对模型大鼠扭体反应及子宫组织内前列腺素含量的影响，探讨不同针刺“得气”状态下部分镇痛机制。方法：按随机数字法，将36只动情间期SD雌鼠分为盐水对照组、寒凝证类痛经模型组（模型组）、刺激量A组（期望“得气”组）与刺激量B组（期望“不得气”组），每组9只。盐水组每日给予生理盐水，其余均采用全身冷冻加雌二醇注射法制备模型，造模成功后刺激量A组采用粗针深刺行手法诱导动物“得气”状态；刺激量B组采用细针浅刺不行手法避免“得气”，观察不同针刺刺激量干预下大鼠每分钟扭体反应，并于留针后20 min取大鼠子宫组织采用酶联免疫吸附法检测PGF2α、PGE2含量的变化。结果：1）每分钟扭体反应评分：与盐水组比较，模型组扭体评分显著增高（P＜0．01）；与模型组比较，期望“得气”组扭体评分显著降低（P＜0．01），与期望“得气”组比较，期望“不得气”组每分钟扭体评分显著增高（P＜0．01）。2）PGF2α、PGE2含量：与盐水组比较，各组PGF2α含量显著增多，期望“不得气”组PGE2含量显著增多（P＜0．01，P＜0．05），模型组与期望“得气”组PGF2α／PGE2比值显著增多。与模型组和期望“得气”组比较，期望“不得气”组PGE2含量增高。结论：针刺三阴交穴可缓解大鼠扭体反应，期望“得气”组疗效显著，期望“不得气”组疗效不及期望“得气”组，相应分子响应机制认为，期望“不得气”组缓解痛经的基础机制可能是通过调节子宫组织内前列腺素E2水平及使PGF2α／PGE2趋于平衡，进而缓解子宫平滑肌痉挛而达到镇痛效果。
목적：관찰불동침자자격량간예삼음교혈후대모형대서뉴체반응급자궁조직내전렬선소함량적영향，탐토불동침자“득기”상태하부분진통궤제。방법：안수궤수자법，장36지동정간기SD자서분위염수대조조、한응증류통경모형조（모형조）、자격량A조（기망“득기”조）여자격량B조（기망“불득기”조），매조9지。염수조매일급여생리염수，기여균채용전신냉동가자이순주사법제비모형，조모성공후자격량A조채용조침심자행수법유도동물“득기”상태；자격량B조채용세침천자불행수법피면“득기”，관찰불동침자자격량간예하대서매분종뉴체반응，병우류침후20 min취대서자궁조직채용매련면역흡부법검측PGF2α、PGE2함량적변화。결과：1）매분종뉴체반응평분：여염수조비교，모형조뉴체평분현저증고（P＜0．01）；여모형조비교，기망“득기”조뉴체평분현저강저（P＜0．01），여기망“득기”조비교，기망“불득기”조매분종뉴체평분현저증고（P＜0．01）。2）PGF2α、PGE2함량：여염수조비교，각조PGF2α함량현저증다，기망“불득기”조PGE2함량현저증다（P＜0．01，P＜0．05），모형조여기망“득기”조PGF2α／PGE2비치현저증다。여모형조화기망“득기”조비교，기망“불득기”조PGE2함량증고。결론：침자삼음교혈가완해대서뉴체반응，기망“득기”조료효현저，기망“불득기”조료효불급기망“득기”조，상응분자향응궤제인위，기망“불득기”조완해통경적기출궤제가능시통과조절자궁조직내전렬선소E2수평급사PGF2α／PGE2추우평형，진이완해자궁평활기경련이체도진통효과。
Objective:To investigate the writhing response of each minute and the synthesis of prostanoids( PGs) of uterine on experimental cold syndrome of primary dysmenorrheal model rats by using different acupuncture methods at SP6.Methods:36 female SD rats were randomized into 4 groups,including saline control group,cold stagnation of primary dysmenorrheal model control group( model control group) ,A stimuli group( Deqi group) and B stimuli group (no Deqi group),with 9 rats in each group.Except the saline control group,in the rest groups rats were treated with the whole body freezing method combined with estradiol benzoate injection mold, and in the saline control group rats were injected 0.9%Nacl.Then the acupuncture groups acupunctured the corresponding point for 20min after modeling,A stimuli group needled bilateral SP6 acupoint with the rats lying face up,in order to induce needing sensation( Deqi);B stimuli group needled the bilateral SP6 acupoint with the rats lying face up,puncturing vertically with one 0.18mm ×13mm stainless,sterilized and disposable to a depth of 1~2mm,maintaining the needle in place for 20 minutes.Observe the writhing response and the PGE2 and PGF2αin uterine of 9 rats in each group by ELISA.Results:Compared with the model control group and B stimuli group,the writhing scores in each minute were decreased significantly in a stimuli group ( P<0 .01 ) . Conclusion:Manual acupuncture at SP6 can relieve the pain of dysmenorrhea and A stimuli group has a better effect than other groups.