CAJ | 학술논문

利用梯度浓度压力驯化法，从厌氧反应器中筛选出对直接红28有具有良好脱色能力的混合菌群 RR。在染料初始浓度为200mg/L，pH=7.0，温度为35℃条件下，经48h 静止培养，染料脱色率可达96.16%。进一步对其培养条件如 pH 值、温度、盐度、初始染料浓度等进行了脱色研究，结果表明，在 pH=7、温度为45℃，盐度为2mmol/L 的情况下，功能菌群脱色效果达到最佳。为了进一步适应工程无机条件，筛选出以染料作为唯一氮源、碳源以及能量的功能菌群，遂将培养基中葡萄糖去掉，同样利用梯度浓度压力驯化法，筛选出混合菌群 RM，并对其脱色性能及群落结构进行分析。混合菌群 RM 在染料初始浓度为50mg/L、温度为35℃、pH=7.0条件下，48h 后其脱色效率为20.05%。利用变性梯度凝胶电泳（DGGE）法，对群落进行分析，混合菌群 RR 主要为伯克霍尔德氏菌属（Burkholderia sp.）、链球菌属（Streptococcus）和克雷伯氏菌属（Klebsiella sp.），菌群 RM 主要为伯克霍尔德氏菌属(Burkholderia sp.)，可见伯克霍尔德氏菌属菌株(Burkholderia sp.)可以适应工程无机环境，并对直接红28存在一定的降解能力。
이용제도농도압력순화법，종염양반응기중사선출대직접홍28유구유량호탈색능력적혼합균군 RR。재염료초시농도위200mg/L，pH=7.0，온도위35℃조건하，경48h 정지배양，염료탈색솔가체96.16%。진일보대기배양조건여 pH 치、온도、염도、초시염료농도등진행료탈색연구，결과표명，재 pH=7、온도위45℃，염도위2mmol/L 적정황하，공능균군탈색효과체도최가。위료진일보괄응공정무궤조건，사선출이염료작위유일담원、탄원이급능량적공능균군，수장배양기중포도당거도，동양이용제도농도압력순화법，사선출혼합균군 RM，병대기탈색성능급군락결구진행분석。혼합균군 RM 재염료초시농도위50mg/L、온도위35℃、pH=7.0조건하，48h 후기탈색효솔위20.05%。이용변성제도응효전영（DGGE）법，대군락진행분석，혼합균군 RR 주요위백극곽이덕씨균속（Burkholderia sp.）、련구균속（Streptococcus）화극뢰백씨균속（Klebsiella sp.），균군 RM 주요위백극곽이덕씨균속(Burkholderia sp.)，가견백극곽이덕씨균속균주(Burkholderia sp.)가이괄응공정무궤배경，병대직접홍28존재일정적강해능력。
A bacterial consortium capable of decoloring Direct Red 28 effectively was screened from a well-running textile printing wastewater biological treatment system,through the method of domestication with gradient concentrations. The bacterial consortium RR had the ability to decolorize Direct Red 28 (at 35℃,pH 7.0,initial dye concentration 200mg/L ) with the decolorization rate being 96.16% in 48h. The optimum pH,temperature,and NaCl concentration for the degradation of Direct Red 28 are 7.0,45℃,and 2mmol/L,respectively. To screen the bacterial consortium RM which fed on Direct Red 28 was to ensure the bacterial consortium to adapt the inorganic conditions. The bacterial consortium RM degrading Direct Red 28 showed the decolorization efficiency of 20.05% (at 35℃,pH 7.0,initial dye concentration 50mg/L ).Through denaturing gradient gel electrophoresis(DGGE) to analyze the composition of microbial communities of the flora RR and RM. DGGE result showed that the dominant groups in the flora RR were Burkholderia bacteria,Streptococcus bacteria and Klebsiella bacteria and the dominant groups in the flora RM was Burkholderia bacteria. The results showed that the Burkholderia bacteria could survive in the inorganic environment and had the ability to degrade the Direct Red 28.