CAJ | 학술논문

建立了高效液相色谱法（HPLC）测定盐酸曲马多口服控释片中盐酸曲马多的含量．采用十八烷基硅烷键合硅胶填充柱（C18柱）为色谱柱，流动相为[醋酸‐醋酸钠缓冲液（p H4．5）]‐甲醇（65∶35），流速为1．0 mL／min ，柱温为30℃，检测波长271 nm ，进样量为20μl ．样品与对照品出峰时间一致，TR≈8．0 min；并且在0．3～0．7 mg／mL浓度范围内线性关系良好，回收率达98％～102％．高效液相色谱法测定盐酸曲马多控释片，简单易行、重复性好．
건립료고효액상색보법（HPLC）측정염산곡마다구복공석편중염산곡마다적함량．채용십팔완기규완건합규효전충주（C18주）위색보주，류동상위[작산‐작산납완충액（p H4．5）]‐갑순（65∶35），류속위1．0 mL／min ，주온위30℃，검측파장271 nm ，진양량위20μl ．양품여대조품출봉시간일치，TR≈8．0 min；병차재0．3～0．7 mg／mL농도범위내선성관계량호，회수솔체98％～102％．고효액상색보법측정염산곡마다공석편，간단역행、중복성호．
The objective of the present study was to establish a high performance liquid chro‐matography (HPLC) determination method of tramadol hydrochloride controlled release tab‐let .An octadecylsilane bonded silica gel packed column (C18 column) was used as the chro‐matographic column .The mobile phase was [acetic acid‐sodium acetate buffer (pH4 .5)]‐methanol (65∶35) ,and the flow rate was 1 .0 mL/min .The column temperature was 30 ℃ , the detection wavelength was 271 nm ,and the injection volume was 20 μl .A consistent peak time between the control and sample was achieved (TR ≈ 8 .0 min) .In the concentration range of 0 .3~0 .7 mg/mL ,the linearity was good .The recovery was measured as 98% ~102% .Results of this work showed that the HPLC determination method of tramadol hydro‐chloride controlled‐release tablet was simple and repeatable .