CAJ | 학술논문

根据形态学特征和ITS序列对分离自黑果枸杞根部的内生真菌RER4进行鉴定，结果该真菌鉴定为米曲霉（Aspergillus oryzae）。采用大米固体培养基对RER4进行发酵，利用多种色谱方法从乙酸乙酯提取物中分离纯化得到8个化合物，借助ESI‐MS、NMR等波谱技术鉴定其结构分别为：麦角甾醇（1）、尿黑酸内酯（2）、6‐柠檬酸甲酯（3）、琥珀酸（4）、1，5‐柠檬酸二甲酯（5）、赤薛醇（6）、甘露醇（7）、阿拉伯糖醇（8）。其中化合物尿黑酸内酯首次从米曲霉发酵物中分离得到。
근거형태학특정화ITS서렬대분리자흑과구기근부적내생진균RER4진행감정，결과해진균감정위미곡매（Aspergillus oryzae）。채용대미고체배양기대RER4진행발효，이용다충색보방법종을산을지제취물중분리순화득도8개화합물，차조ESI‐MS、NMR등파보기술감정기결구분별위：맥각치순（1）、뇨흑산내지（2）、6‐저몽산갑지（3）、호박산（4）、1，5‐저몽산이갑지（5）、적설순（6）、감로순（7）、아랍백당순（8）。기중화합물뇨흑산내지수차종미곡매발효물중분리득도。
The fungus strain RER4 isolated from the root of Lycium ruthenicum .Was identi‐fied as A spergillus oryz ae by its morphological characteristics and internal transcrised spacer (ITS) sequence analyses .Solid state fermentation was used to culture the endophytic fungi RER4 on the rice medium ,8 compounds were isolated and purified from the eyhyl acetate ex‐tract of endophytic fungi RER4 by various chromatographic methods and their structures were ergosterol ,5‐hydroxy‐benzofuran‐2 (3H )‐one ,3‐hydroxy‐3‐(methoxycarb onyl) pen‐tanedi‐oic acid ,succinic acid ,2‐hydroxy‐4‐methoxy‐2‐(2‐meth oxy‐2‐oxoethyl )‐4‐oxobutan‐oic acid ,erythritol ,mannitol ,adonitol by ESI‐MS and NMR .Among compound 5‐hydroxy‐benzofuran‐2(3H)‐one was firstly isolated from the medium of Aspergillus oryzae .