CAJ | 학술논문

为获得银杏基因组DNA 的最佳提取方法，本文以银杏嫩叶为研究对象，利用传统CTAB法、改良CTAB法、改良SDS法等方法提取银杏基因组DNA ，进而利用凝胶电泳法、紫外分光光度法、PCR扩增等方法分析提取效率和质量，确定最适合银杏基因组DNA的提取方法。结果表明，改良CTAB法对银杏基因组DNA提取效果最佳，为3种方法中最适合提取银杏基因组DNA的方法。
위획득은행기인조DNA 적최가제취방법，본문이은행눈협위연구대상，이용전통CTAB법、개량CTAB법、개량SDS법등방법제취은행기인조DNA ，진이이용응효전영법、자외분광광도법、PCR확증등방법분석제취효솔화질량，학정최괄합은행기인조DNA적제취방법。결과표명，개량CTAB법대은행기인조DNA제취효과최가，위3충방법중최괄합제취은행기인조DNA적방법。
In order to obtain a suitable extraction method of genomic DNA from Ginkgo biloba leaves ,here the method of traditional CTAB ,improved CTAB method ,improved SDS method were investigated to extract genomic DNA of Ginkgo biloba .To assess the quality of DNA extraction ,gel electrophoresis ,UV spectrophotometry and PCR amplification were used to analyze the genomic DNA of Ginkgo biloba L .The results show that the quantity and the quality of the DNA extracted by the improved CTAB is high enough to perform PCR hundreds of PCR‐based reactions and also to be used in other DNA manipulation techniques .