贵阳医学院学报
貴暘醫學院學報
귀양의학원학보
JOURNAL OF GUIYANG MEDICAL COLLEGE
2015年
6期
595-599
,共5页
陈渔%胡珊%王彦青%王林%王建光
陳漁%鬍珊%王彥青%王林%王建光
진어%호산%왕언청%왕림%왕건광
电针%酒精%抑郁%Toll样受体%炎性细胞因子%IL-1β%IL-6%TNF-α
電針%酒精%抑鬱%Toll樣受體%炎性細胞因子%IL-1β%IL-6%TNF-α
전침%주정%억욱%Toll양수체%염성세포인자%IL-1β%IL-6%TNF-α
electroacupuncture%ethanol%depression%Toll like receptor%inflammatory cytokine%IL-1β%IL-6%TNF-α
目的：观察电针对急性酒精灌胃后抑郁大鼠海马TLR2/4及炎性细胞因子表达的影响。方法：将48只SD雄性大鼠，随机分成2组，为生理盐水对照组（ Control，生理盐水灌胃）和酒精模型组（ Ethanol，50％酒精5 g/kg灌胃），通过强迫游泳实验和旷场实验观察两组的行为学改变，若出现抑郁样症状提示造模成功；再随机从Control组和Ethanol组各抽取12只大鼠，为生理盐水加电针组（ Control﹢EA）和酒精模型加电针组（ Ethanol﹢EA），均予电针刺激（1次/d），分别于实验24 h和72 h取大鼠海马组织，用实时荧光定量逆转录聚合酶链反应检测其中Toll样受体2/4（TLR2/4）、白介素-1β（IL-1β）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）mRNA的表达。结果：急性酒精灌胃24 h和72 h后，与Control组比较，Ethanol组大鼠TLR2/4、IL-1β、IL-6、TNF-αmRNA的表达明显增加，差异有统计学意义（ P＜0.05），Control﹢EA组无明显变化，差异无统计学意义（ P＞0.05）；与Ethanol组相比，Ethanol﹢EA组 TLR2/4、IL-1β、IL-6、TNF-α mRNA 的表达显著减少，差异有统计学意义（ P ＜0.05）；Control﹢EA与Ethanol﹢EA两组间比较，差异无统计学意义（ P＞0.05）。结论：电针能下调急性饮酒后24 h、72 h抑郁样行为大鼠海马组织中TLR2/4、IL-1β、IL-6、TNF-αmRNA的表达，这可能参与了电针抗抑郁的机制。
目的：觀察電針對急性酒精灌胃後抑鬱大鼠海馬TLR2/4及炎性細胞因子錶達的影響。方法：將48隻SD雄性大鼠，隨機分成2組，為生理鹽水對照組（ Control，生理鹽水灌胃）和酒精模型組（ Ethanol，50％酒精5 g/kg灌胃），通過彊迫遊泳實驗和曠場實驗觀察兩組的行為學改變，若齣現抑鬱樣癥狀提示造模成功；再隨機從Control組和Ethanol組各抽取12隻大鼠，為生理鹽水加電針組（ Control﹢EA）和酒精模型加電針組（ Ethanol﹢EA），均予電針刺激（1次/d），分彆于實驗24 h和72 h取大鼠海馬組織，用實時熒光定量逆轉錄聚閤酶鏈反應檢測其中Toll樣受體2/4（TLR2/4）、白介素-1β（IL-1β）、白介素-6（IL-6）、腫瘤壞死因子-α（TNF-α）mRNA的錶達。結果：急性酒精灌胃24 h和72 h後，與Control組比較，Ethanol組大鼠TLR2/4、IL-1β、IL-6、TNF-αmRNA的錶達明顯增加，差異有統計學意義（ P＜0.05），Control﹢EA組無明顯變化，差異無統計學意義（ P＞0.05）；與Ethanol組相比，Ethanol﹢EA組 TLR2/4、IL-1β、IL-6、TNF-α mRNA 的錶達顯著減少，差異有統計學意義（ P ＜0.05）；Control﹢EA與Ethanol﹢EA兩組間比較，差異無統計學意義（ P＞0.05）。結論：電針能下調急性飲酒後24 h、72 h抑鬱樣行為大鼠海馬組織中TLR2/4、IL-1β、IL-6、TNF-αmRNA的錶達，這可能參與瞭電針抗抑鬱的機製。
목적：관찰전침대급성주정관위후억욱대서해마TLR2/4급염성세포인자표체적영향。방법：장48지SD웅성대서，수궤분성2조，위생리염수대조조（ Control，생리염수관위）화주정모형조（ Ethanol，50％주정5 g/kg관위），통과강박유영실험화광장실험관찰량조적행위학개변，약출현억욱양증상제시조모성공；재수궤종Control조화Ethanol조각추취12지대서，위생리염수가전침조（ Control﹢EA）화주정모형가전침조（ Ethanol﹢EA），균여전침자격（1차/d），분별우실험24 h화72 h취대서해마조직，용실시형광정량역전록취합매련반응검측기중Toll양수체2/4（TLR2/4）、백개소-1β（IL-1β）、백개소-6（IL-6）、종류배사인자-α（TNF-α）mRNA적표체。결과：급성주정관위24 h화72 h후，여Control조비교，Ethanol조대서TLR2/4、IL-1β、IL-6、TNF-αmRNA적표체명현증가，차이유통계학의의（ P＜0.05），Control﹢EA조무명현변화，차이무통계학의의（ P＞0.05）；여Ethanol조상비，Ethanol﹢EA조 TLR2/4、IL-1β、IL-6、TNF-α mRNA 적표체현저감소，차이유통계학의의（ P ＜0.05）；Control﹢EA여Ethanol﹢EA량조간비교，차이무통계학의의（ P＞0.05）。결론：전침능하조급성음주후24 h、72 h억욱양행위대서해마조직중TLR2/4、IL-1β、IL-6、TNF-αmRNA적표체，저가능삼여료전침항억욱적궤제。
Objective:To investigate the effect of electroacupuncture on TLR2/4 and pro-inflammato-ry cytokine expression in hippocampus of rats with acute ethanol intragastric administration. Method:Forty-eight SD male rats were randomly divided into two groups:control group( rats were given normal saline by gavage)and acute ethanol model group( rats were given 50% ethanol 5 g/kg by gavage). Behavioral changes of rats were observed with forced swimming and open field test,and depression-like symptoms suggested that the model was successful. Then 12 rats from each group were randomly select-ed as control plus electroacupuncture( EA)stimulation group and ethanol plus EA stimulation group, and the rats were accepted EA stimulation once a day. The hippocampus tissues were obtained at 24 h and 72 h after experiment,the expressions of TLR2/4,IL-1β,IL-6 and TNF-α mRNA were deter-mined with QRT-PCR. Results:Compared with the control group,the expressions of TLR2/4,IL-1β, IL-6 and TNF-α mRNA in hippocampus of ethanol model rats increased at 24 h and 72 h after acute ethanol intragastric administration( P<0 . 05 ),yet the differences in the control﹢EA group were not significant(P>0. 05). Compared with ethanol group,the expressions of TLR2/4,IL-1β,IL-6 and TNF-α mRNA in ethanol﹢EA treatment group significantly decreased(P<0. 05). However,no sta-tistical difference was found between control﹢EA group and ethanol﹢EA group. Conclusion:Elec-troacupuncture can decrease TLR2/4 ,IL-1β,IL-6 and TNF-α mRNA expression in hippocampus rats with acute ethanol intragastric administration. It may be associated with the mechanism of the antide-pressive effects of EA.
