粮食与饲料工业
糧食與飼料工業
양식여사료공업
CEREAL & FEED INDUSTRY
2015年
6期
51-55
,共5页
宫晓%郑喜群%刘晓兰%邓永平
宮曉%鄭喜群%劉曉蘭%鄧永平
궁효%정희군%류효란%산영평
好食脉孢菌%固态发酵%木聚糖酶%培养条件
好食脈孢菌%固態髮酵%木聚糖酶%培養條件
호식맥포균%고태발효%목취당매%배양조건
Neurospora sitophila%solid state fermentation%xylanase%culture conditions
研究了好食脉孢菌固体发酵生产木聚糖酶的条件,以提高其产量。通过单因素及Plackett‐Burman设计法,初步选出生产木聚糖酶适合的培养条件和无机盐种类。结果表明:培养基主要物料是豆饼粉、玉米皮,比例为4∶1,无机盐为0.3%硫酸铜与0.6%氯化钙,发酵时间为60h,浸提时间为4h,在此优化条件下,木聚糖酶活力可达577 U/m l。
研究瞭好食脈孢菌固體髮酵生產木聚糖酶的條件,以提高其產量。通過單因素及Plackett‐Burman設計法,初步選齣生產木聚糖酶適閤的培養條件和無機鹽種類。結果錶明:培養基主要物料是豆餅粉、玉米皮,比例為4∶1,無機鹽為0.3%硫痠銅與0.6%氯化鈣,髮酵時間為60h,浸提時間為4h,在此優化條件下,木聚糖酶活力可達577 U/m l。
연구료호식맥포균고체발효생산목취당매적조건,이제고기산량。통과단인소급Plackett‐Burman설계법,초보선출생산목취당매괄합적배양조건화무궤염충류。결과표명:배양기주요물료시두병분、옥미피,비례위4∶1,무궤염위0.3%류산동여0.6%록화개,발효시간위60h,침제시간위4h,재차우화조건하,목취당매활력가체577 U/m l。
The solid state fermentation conditions of xylanase production by Neurospora sitophila was studied ,in order to improve the yield .Through single factors and Plackett‐Burman design method ,the optimum culture conditions and inorganic salt types were selected .The results showed that the suitable culture medium was composed of soybean powder and corn bran with the ratio of 4∶1 ;inorganic salt was copper sulfate and calcium chloride with concentration of 0 .3% and 0 .6% ,respec‐tively ;fermentation time was 60 h and extraction time was 2 h . Under the conditions ,the activity of xylanase reached 577 U/m l .